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master's thesis
Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:696021
Pšeničnik, Silvia
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Pšeničnik, S. (2019). Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:696021

Pšeničnik, Silvia. "Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:696021

Pšeničnik, Silvia. "Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:696021

Pšeničnik, S. (2019). 'Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:696021

Pšeničnik S. Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:696021

S. Pšeničnik, "Ispitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:696021

Metadata
TitleIspitivanje interakcija derivata cijanina i piperazina s dvolančanim DNA i RNA spektroskopskim metodama
Title (english)Study of interactions of cyanine-piperazine derivatives with double-stranded DNA and RNA by spectroscopic methods
AuthorSilvia Pšeničnik
MentorLidija-Marija Tumir (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberInes Primožič (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U okviru ovog diplomskog rada spektroskopski su okarakterizirane vodene otopine šest novosintetiziranih cijaninskih boja koje sadržavaju benzotiazolnu podjedinicu i metilpiperazinsku, odnosno fenilpiperazinsku podjedinicu. Ispitane su njihove interakcije s dvolančanim polinukleotidima. Interakcije cijaninskih boja s različitim polinukleotidima ispitane su koristeći standardne spektrofotometrijske metode UV/Vis-spektroskopije, termičke denaturacije, fluorimetrije i CD spektroskopije. Otopine spojeva imaju UV/Vis spektar u području od 220 nm do 600 nm i velike molarne ekstincijske koeficijente, a slabi intenzitet fluorescencije spojeva značajno se povećao dodatkom polinukleotida. Ispitani spojevi su akiralni te nemaju CD spektar. Novosintetizirani derivati različito se vežu za polinukleotide različite sekundarne strukture, uz odgovarajuće različite intenzitete fluorescencije. Općenito, fenilpiperazinski spojevi pokazuju slabije vezanje od metilpiperazinskih spojeva. Spektrofotometrijska ispitivanja upućuju većinom na vezanje cijaninske boje u utore DNA/RNA, no kod fenilpiperazinskih derivata može se pretpostaviti interkalativni način vezanja.
Abstract (english)
In the scope of thesis water solution of six newly synthesized cyanine dyes containing the benzothiazole subunit and the methylpiperazine or phenylpiperazine subunits were characterized spectroscopicaly. Their interactions with double-stranded polynucleotides were studied. The interactions of the conjugates with different polynucleotides were studied using standard spectroscopic methods of UV/Vis spectroscopy, thermal denaturation experiments, fluorescence and CD spectroscopy. Aqueous solutions of compounds absorbed in the UV/Vis region 220-600 nm and had a high molar extinction coefficients. Compounds had a large increase in fluorescence intensity upon addition of polynucleotides. Aqueous solutions of compounds had no intrinsic CD spectrum. Novel cyano dyes, although intrinsically non-fluorescent, give rise to strong fluorimetric response upon binding to double-stranded (ds)-DNAs or (ds)-RNA, whereby selectivity in emission response to various polynucleotide secondary structures is controlled by linker length. In general, phenylpiperazine compounds show weaker binding than metylpiperazine compounds. Mostly, new compounds bind to the polynucleotide minor groove, while in case of phenyl-piperazine derivatives intercalation into DNA/RNA was observed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:696021
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-03-24
Terms of useLicence In copyright icon
Created on2019-09-27 09:03:58