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master's thesis
Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:141563
Pavlak, Lucija
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Pavlak, L. (2019). Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:141563

Pavlak, Lucija. "Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:141563

Pavlak, Lucija. "Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:141563

Pavlak, L. (2019). 'Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:141563

Pavlak L. Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 May 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:141563

L. Pavlak, "Osjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:141563

Metadata
TitleOsjetljivost izoleucil-tRNA-sintetaza iz različitih organizama prema mupirocinu na proteinskoj i staničnoj razini
Title (english)Mupirocin sensitivity of isoleucyl-tRNA synthetases from various organisms on protein and cellular level
AuthorLucija Pavlak
MentorMorana Dulić (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberJana Pisk (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Izoleucil-tRNA-sintetaza (IleRS) kovalentno veže izoleucin na pripadnu tRNAIle, pri čemu ponekad griješi. Radi toga posjeduje mehanizme popravka vlastite pogreške. Uočena su 2 tipa IleRS koji se strukturno razlikuju u C-terminalnoj domeni. IleRS1 se nalazi kod prokariota, dok se IleRS2 pojavljuje kod eukariota, ali i kod nekih prokariota. IleRS1 je, u pravilu, osjetljiviji na mupirocin od IleRS2 i uz popravak pogreške nakon prijenosa koristi i tRNAovisni popravak misaktivirane aminokiseline, koji nije detektiran na dosad istraživanim IleRS2. U ovom radu želi se preispitati korelacija između osjetljivosti prema mupirocinu i tipa IleRS-a iz više različitih organizama. Uklonirana su 3 gena za IleRS, te je eksprimiran jedan protein, koji će se u daljnjem radu testirati na inhibiciju mupirocinom. Inhibicija rasta bakterijskih stanica (izražena kao minimalna inhibitorna koncentracija (MIC)) mupirocinom ne pokazuje strogo korelaciju s tipom IleRS-a, što može biti posljedica različitog unosa mupirocina u stanice različitih organizama.
Abstract (english)
Isoleucyl-tRNA synthetase (IleRS) covalently binds isoleucine to their cognate tRNAIle, sometimes making mistakes. Therefore, it acquired editing activity to maintain overall accuracy of translation. Two types of IleRS have been observed that differ structurally in the C-terminal domain. IleRS1 is found in prokaryotes, while IleRS2 occurs in eukaryotes as well as in some prokaryotes. Generally, IleRS1 is more sensitive to mupirocin than IleRS2, and in addition to post-transfer editing, uses a tRNA-dependent pretransfer editing, which was not detected on the IleRS2 studied so far. Here we aim to examine the correlation between mupirocin sensitivity and type IleRS from a number of different organisms. In this work, 3 genes coding for IleRS were cloned and one protein was expressed and purified, which will be assayed in vitro for mupirocin inhibition. The inhibition of bacterial cell growth (expressed as minimum inhibitory concentration (MIC)) by mupirocin does not show a strict correlation with the type of IleRS, which may be due to different uptake of mupirocin into cells by different organisms.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:141563
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-09-20
Terms of useLicence In copyright icon
Created on2019-09-27 10:02:22