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master's thesis
Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:421375
Košćak, Marta
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Košćak, M. (2019). Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:421375

Košćak, Marta. "Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:421375

Košćak, Marta. "Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:421375

Košćak, M. (2019). 'Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA', Master's thesis, University of Zagreb, Faculty of Science, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:421375

Košćak M. Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:217:421375

M. Košćak, "Konjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:421375

Metadata
TitleKonjugati cijaninske boje i gvanidinijkarbonil-pirola kao nove fluorescentne i CD probe za razlikovanje različitih sekundarnih struktura dvolančane DNA ili dvolančane RNA
Title (english)The conjugates of cyanine dye and guanidiniocarbonyl-pyrrole as a fluorescent and CD probes for distinguishing between various double stranded DNA or double stranded RNA secondary structures
AuthorMarta Košćak
MentorIvo Piantanida (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberInes Primožič (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberNives Galić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-09-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U sklopu rada okarakterizirana su dva nova konstitucijska izomera gvanidinijkarbonil-pirola (GCP) i 6-bromo-tiazol oranž (cijanin), koji se razlikuju u svojoj povezanosti: kod jednog konjugata GCP je s cijaninom vezan preko atoma dušika u benzotiazolu, a drugi konjugat je povezan preko dušika u kinolinu. Spojevi pokazuju različita svojstva u vodenom mediju, kao i različitost u prepoznavanju raznih dvolančanih DNA i dvolančanih RNA. Specifična karakteristika cijanina je povećana sklonost agregiranja u vodenom mediju. Oba spoja se efikasno vežu na dvolančane DNA i RNA s mikromolarnim afinitetom, s tim da je benzotiazolni izomer pokazao izrazitu fluorescentnu selektivnost prema dvolančanoj RNA, u usporedbi s ostalim ispitivanim dvolančanim DNA uzvojnicama, dok je kinolinski analog pokazao slabu fluorescencijsku selektivnost prema dvolančanoj GC-DNA. Vezanjem na DNA ili RNA, samo je izomer povezan preko kinolina pokazao, po vezanju na DNA/RNA, pojavu induciranog cirkularnog dikroizma (ICD; λ=450-550 nm), s tim da je pomoću ICD vrpci moguće pouzdano razlikovati A-T(U) polinukleotide od G-C polinukleotida.
Abstract (english)
Two novel constitutional isomers of guanidiniocarbonyl-pyrrole (GCP) and 6-bromo-thiazole orange (cyanine) were characterized, differing only in linker connectivity between GCP and cyanine (benzothiazole nitrogen vs quinoline nitrogen). Conjugates revealed distinctively different properties in aqueous medium, as well as different recognition pattern of various ds-DNA or ds-RNA sequences. Namely, quinolone-linked was more prone to aggregation in aqueous medium. Both compounds effectively bind, with micromolar affinity, to double-stranded DNA and RNA, whereby benzothiazole-linked isomer showed distinct fluorimetric selectivity toward ds-RNA in comparison to any other tested ds-DNA, whereas the quinolone-linked analog showed similar fluorescence response to all studied DNA/RNA. By binding to DNA or RNA, only the quinoline analogue showed induced circular dichroism bands (ICD; λ = 450-550 nm), whereby it was possible to use an ICD bands to reliably differentiate AT (U) polynucleotides from GC polynucleotides.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:421375
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-09-06
Terms of useLicence In copyright icon
Created on2019-09-30 11:04:53