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master's thesis
Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:918142
Badovinac, Marko
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Badovinac, M. (2019). Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:918142

Badovinac, Marko. "Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:918142

Badovinac, Marko. "Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:918142

Badovinac, M. (2019). 'Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:918142

Badovinac M. Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:918142

M. Badovinac, "Spektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:918142

Metadata
TitleSpektroskopska karakterizacija dipeptida pirena i cijanina i njihove interakcije s dvolančanim polinukleotidima
Title (english)Spectroscopic characterization of pyrene-cyanine dipeptides and their interactions with double-stranded polynucleotides
AuthorMarko Badovinac
MentorIvo Piantanida (mentor)
Committee memberGordan Horvat (predsjednik povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
Committee memberDavor Kovačević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Pireni i cijanini poznate su fluorescentne probe. Pireni se često koriste za praćenje konformacijskih promjena proteina kovalentno vezanih na njih dok se cijanini, intrinzično nefluorescentni, odlikuju izrazitom fluorescencijom prilikom vezanja na biološke mete kao što su proteini ili nukleinske kiseline. U sklopu ovog diplomskog rada proučavana su dva novosintetizirana i do sad nepoznata izomera dipeptida pirena i cijanina. Prvo su ispitana njihova spektroskopska svojstva, temperaturna stabilnost te uvjeti agregiranja UV/Vis spektroskopijom. Potom su ispitane interakcije dvaju izomera s dvolančanim polinukleotidima različitih slijedova baza i sekundarnih struktura. Obradom fluorimetrijskih titracija dobivene su konstante stabilnosti kompleksa s polinukleotidima te ovisnost intenziteta emisije o sekundarnoj strukturi polinukleotida. Način vezanja spojeva na polinukleotide određen je spektroskopijom cirkularnog dikroizma, dok je utjecaj na stabilizaciju dvolančane uzvojnice određen spektroskopski određivanjem temperature mekšanja kompleksa. Dobiveni rezultati ukazuju na biološki relevantne interakcije novih spojeva s DNA i RNA.
Abstract (english)
Pyrenes and cyanines are known fluorescent probes. Pyrenes are often used to monitor the conformational changes of proteins by covalently bound to them, whereas cyanins, intrinsically nonfluorescent, are characterized by a pronounced fluorescence emission when bound to biological targets such as proteins or nucleic acids. Two newly synthesized and still unknown isomers of pyrene and cyanine dipeptides were studied as part of this thesis. First, their spectroscopic properties, temperature stability, and aggregation conditions by UV/Vis spectroscopy were examined. The interactions of the two isomers with double-stranded polynucleotides of different base sequences and secondary structures were then examined. Treatment with fluorimetric titrations obtained the stability constants of the complex with polynucleotides and the dependence of the emission intensity on the secondary structure of the polynucleotides. The mode of binding compounds to polynucleotides was determined by spectroscopy of circular dichroism, while the influence on stabilization of the double-stranded helix was determined spectroscopically by determining the melting temperature of the complex. The results obtained indicate the biologically relevant interactions of new compounds with DNA and RNA.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:918142
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-09-30 12:31:03