prikaz prve stranice dokumenta Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster
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master's thesis
Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:470397
Pulitika, Anamarija
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Pulitika, A. (2019). Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:470397

Pulitika, Anamarija. "Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:470397

Pulitika, Anamarija. "Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:470397

Pulitika, A. (2019). 'Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:470397

Pulitika A. Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:470397

A. Pulitika, "Utjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:470397

Metadata
TitleUtjecaj hladnog stresa i bakterijskih infekcija na ekspresiju satelitskih DNA u kukcima Tribolium castaneum i Drosophila melanogaster
Title (english)Influence of cold stress and bacterial infection on satellite DNA expression in insects Tribolium castaneum and Drosophila melanogaster
AuthorAnamarija Pulitika
MentorĐurđica Ugarković (mentor)
Committee memberJasmina Rokov Plavec (predsjednik povjerenstva)
Committee memberBranimir Bertoša (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Satelitsku DNA čine uzastopno ponavljajući sljedovi DNA koji se uglavnom nalaze u centromernom, pericentromernom i telomernom dijelu heterokromatina i zauzimaju značajan dio genoma u eukariotskom organizmu. U brašnaru Tribolium castaneum, okarakterizirane su glavna (TCAST1) i sporedna satelitska DNA (TCAST2) čiji su elementi pronađeni raspršeni unutar eukromatina u blizini jedinstvenih gena. U vinskoj mušici Drosophila melanogster, okarakterizirana je glavna satelitska DNA (1.688) čiji su elementi također raspršeni unutar eukromatina. Transkripti satelitskih DNA procesiraju se u male interferirajuće molekule RNA (siRNA) koje mogu imati utjecaj na epigenetsko stanje heterokromatina. Dokazano je da je ekspresija satelitskih elemenata TCAST osjetljiva na temperaturu, što upućuje na to da je moguća njihova uloga u regulaciji ekspresije gena pod utjecajem stresa. Hladni stres, za razliku od toplinskog, nije pokazao utjecaj na ekspresiju satelitskih DNA. Bakterijske infekcije mogu dovesti do smanjenja ekspresije satelitskih DNA, ovisno o načinu infekcije, prethodnoj imunizaciji i vrsti bakterije.
Abstract (english)
Satellite DNA are tandemly repeated DNA sequences that constitute a considerable part of eukaryotic genome and are mostly present in centromeric, pericentromeric and subtelomeric regions of heterochromatin. In the beetle Tribolium castaneum, major (TCAST1) and minor (TCAST2) satellite DNA whose elements are found dispersed in the vicinity of unique genes within euchromatin are characterized. In the fruit fly Drosophila melanogaster, elements of major satellite DNA (1.688) are also found dispersed within euchromatin. Transcripts of satellite DNA are processed into small interfering RNA (siRNA) that might affect the epigenetic state of heterochromatin. Expression of elements of satellite DNA seems to be highly sensitive to temperature and might play a role in the regulation of gene expression under stress condition. Unlike heat shock, cold stress did not show effect on satellite expression. Bacterial infection may lead to decreased expression of satellite DNA, but it depends on many factors, such as infection routes, priming or bacterial species.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:470397
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-09-30
Terms of useLicence In copyright icon
Created on2019-10-02 12:18:56