Springs represent ecotons, i.e. the transition zone where groundwater rises to the surface of the earth forming surface flow. Karst springs are specific type of springs. Permanent karst springs represent stable habitats with very low seasonal variations of abiotic conditions, while temporary springs show more pronounced seasonal oscillations. In this study we explored the macroinvertebrate communities of four different karst springs, three of them were permanent (springs of the river Krka, Krupa and Kosovčica) and one temporary (Krčić spring). One-year research was conducted with monthly macroinvertebrates sampling using Surber sampler. Seasonal fluctuations in physico-chemical parameters of the water were generally low, nevertheless there were differences between the studied springs, depending on the origin of water. The greatest differences in the composition, structure and seasonal variations of macroinvertebrates were observed between permanent and temporary springs, indicating the importance of the presence of the surface flow. Overall, diversity and abundance of macroinvertebrates were higher at the permanent springs than the Krčić spring, which dries up in the summer months. In permanent springs, greatest abundance of macroinvertebrates was recorded in moss, whereas at the Krčić spring it was highest in the mesolithal, due to the high density of Micropterna nycterobia (Limnephilidae, Trichoptera). This species has specifically adapted life cycle to the seasonal drying up, and large proportion of such species is typical for communities in temporary habitat.