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master's thesis
Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814)
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:007603
Jarak, Matea
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Jarak, M. (2013). Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:007603

Jarak, Matea. "Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814)." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:007603

Jarak, Matea. "Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814)." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:007603

Jarak, M. (2013). 'Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814)', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:007603

Jarak M. Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:007603

M. Jarak, "Diferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:007603

Metadata
TitleDiferencijalna i citokemijska analiza krvnih stanica babice balavice (Parablennius sanguinolentus, Pallas 1814)
Title (english)Differential and Cytochemical Analysis of Blood Cells in Rusty Blenny (Parablennius sanguinolentus, Pallas 1814)
AuthorMatea Jarak
MentorDomagoj Đikić (mentor)
Committee memberDomagoj Đikić (predsjednik povjerenstva)
Committee memberSven Jelaska (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Rad opisuje hematološku analizu pune krvi babice balavice Parablennius sanguinolentus. Utvrđena je brojnost eritrocita, trombocita, limfocita, neutrofila, monocita i bazofila. Morfološka i kvantitativna analiza krvnih stanica provedena je bojanjem May-Grünwald Giemsom. Diferencijalnim citokemijskim tehnikama napravljena je citološka karakterizacija razlicitih tipova stanica. Glikokonjugati i polisaharidi dokazani su tehnikama alcian blue (AB) i periodic acid Schiff (PAS). Stanicni lipidi dokazani su tehnikom sudan black B (SBB). Enzimatsko-citokemijskim tehnikama dokazala se aktivnost alfa-naftil acetat esteraza (ANAE), alfa-naftil butirat esteraza (ANBE) te alkalne fosfataze (ALP). Brojnost i udio leukocita, eritrocita i morfologija stanica slicni su ostalim vrstama iz reda Perciformes. Pozitivnu reakciju na bojanje ABom imaju jezgre svih stanica. PASom se boje limfociti, monociti, neutrofili i trombociti. SBBom se boje limfociti, neutrofili i monociti. Aktivnost ANAE nije pokazao niti jedan tip stanice. Aktivnost ANBE pokazuju limfociti, neutrofili i monociti. APFB je pokazala aktivnost kod neutrofila i monocita.
Abstract (english)
This thesis presents results attained from peripheral blood of the fish Parablennius sanguinolentus. It contains erythrocyte, thrombocyte, limphocyte, monocyte, neutrophile and basophile counts. The May-Grünwald Giemsa stain was used in morphological and quantitative blood cell analysis. Cytological characterization of all blood cell types used differential cytochemical stains. The alcian blue (AB) dye and the periodic acid Schiff method (PAS) stained glycoconjugates and polysacharides. The sudan black B (SBB) dye stained cell lipids. Activities of alpha-naphthyl acetate esterase (ANAE), alpha-naphthyl butyrate esterase (ANBE) and alkaline phosphatase (ALP) were proved using enzyme-cytochemical reactions. Leukocyte and erithrocyte count, percentage and cell morphology are similar to other fish species from order Perciformes. AB stained nuclei of all cells. PAS stained lymphocytes, monocytes, neutrophils and thrombocytes. SBB stained lymphocytes, neutrophils and monocytes. ANAE did not show reaction in any type of cell. ANBE stained lymphocytes, neutrophils and monocytes. APFB showed reactions in neutrophiles and monocytes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:007603
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-04-21 10:55:48