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master's thesis
Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:439350
Kokotović, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Kokotović, I. (2019). Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:439350

Kokotović, Iva. "Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:439350

Kokotović, Iva. "Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:439350

Kokotović, I. (2019). 'Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:439350

Kokotović I. Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:439350

I. Kokotović, "Procjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:439350

Metadata
TitleProcjena toksičnosti vode rijeke Save upotrebom običnog luka (Allium cepa L.) kao modelnog organizma
Title (english)Toxicity assessment of Sava river using onion (Allium cepa L.) as a model organism
AuthorIva Kokotović
MentorSandra Radić Brkanac (mentor)
Committee memberSandra Radić Brkanac (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Povećana urbanizacija i otpuštanje otpadnih voda u rijeke dovodi do sve većeg broja organskih zagađivača u vodenim ekosustavima i potrebe za pročišćavanjem otpadnih voda. Kvaliteta vode rijeke Save, uzorkovane na četiri lokacije (Lukavec, Rugvica, Jesenice i Hrušćica), u ovom je radu procijenjena pomoću Allium-testa i biokemijskih parametara - sadržaja proteina, karbonila i malondialdehida te aktivnosti antioksidacijskih enzima (superoksid dismutaze, askorbat peroksidaze, katalaze i glutation-S-transferaze). Kao modelni organizam korišten je obični luk (Allium cepa L.), koji je pogodan za istraživanje cito- i genotoksičnosti zbog malog broja dugačkih kromosoma (2n = 16). Uzorci vode rijeke Save nisu inhibirali rast korjenčića luka, no pokazali su se genotoksičnim, a uzorak s lokacije Jesenice i citotoksičnim. Rezultati aktivnosti antioksidacijskih enzima i karbonilacije proteina pokazuju da su svi uzorci vode rijeke Save izazvali oksidacijski stres u korjenčićima luka i posljedično uzrokovali oštećenja na proteinima. Zbog jednostavnog načina čuvanja i rukovanja te osjetljivosti, obični luk je pogodan modelni organizam za biomonitoring površinskih voda.
Abstract (english)
Increased urbanization and disposal of wastewater into rivers leads to ever-growing number of organic contaminants in aquatic ecosystems and the need for wastewater treatment. In the present study, the quality of Sava River collected at four locations (Lukavec, Rugvica, Jesenice i Hrušćica) has been evaluated using Allium-test and certain biochemical parameters – contents of proteins, malondialdehide and carbonyls as well as activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, catalase and glutathione-S-transferase). Onion (Allium cepa L.) is often used as a model organism in cyto- and genotoxic studies because it has a small number of relatively large chromosomes (2n = 16). Samples of Sava River did not cause growth inhibition of onion roots, but they were genotoxic and the sample Jesenice was cytotoxic as well. The results of antioxidative enzyme activities and protein carbonilation showed that all samples of Sava River cause oxidative stress in onion roots and consequently damage to proteins. Due to the simplicity and low cost of the procedure as well as its sensitivity, onion is a suitable model organism for biomonitoring of surface waters.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:439350
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-09-19
Terms of useLicence In copyright icon
Created on2019-10-09 11:23:18