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master's thesis
Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:603485
Mešin, Mario
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Mešin, M. (2015). Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:603485

Mešin, Mario. "Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:603485

Mešin, Mario. "Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:603485

Mešin, M. (2015). 'Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:603485

Mešin M. Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:603485

M. Mešin, "Raznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:603485

Metadata
TitleRaznolikost gena skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz Izraela
Title (english)Variability of major histocompatibility complex class II genes in the golden jackals (Canis aureus) from Israel
AuthorMario Mešin
MentorAna Galov (mentor)
Committee memberAna Galov (predsjednik povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
Committee memberAntun Alegro (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Geni glavnog sustava tkivne podudarnosti (MHC) ključna su komponenta imunološkog sustava kod sisavaca te su i važan molekularni marker adaptivne genetičke raznolikosti u divljim populacijama životinja. Veća raznolikost MHC gena unutar populacije povećava izglede populacije za obranu od patogena i parazita, a time i izglede za dugoročno preživljavanje. Čagalj (Canis aureus) pripada porodici Canidae (psi), podred Caniformia, redu Carnivora (mesojedi). Do sada nema objavljenih istraživanja raznolikosti MHC gena ove vrste. Cilj ovog diplomskog rada je odrediti razinu raznolikosti DQA, DQB i DRB gena skupine II MHC sustava u čagljeva iz Izraela. Iz 30 uzoraka uške čaglja, postupcima izolacije DNA, PCR metodom, elektroforezom, sekvenciranjem i molekularnim kloniranjem, pronađena su tri alela na DQA lokusu, pet alela na DQB lokusu i četiri alela na DRB lokusu. Našli smo jedan novi jedinstveni alel na DRB lokusu (DRB1*04802var). Ostali pronađeni aleli poznati su iz prethodnih istraživanja na ostalim vrstama porodice pasa, što potvrđuje postojanje trans-specijskog polimorfizma. DQB i DRB lokusi pokazuju veliku raznolikost alela i velike evolucijske udaljenosti te visok omjer nesinonimnih supstitucija naspram sinonimnih, što potvrđuje pretpostavku djelovanja pozitivne selekcije na te lokuse.
Abstract (english)
The genes of major histocompatibility (MHC) system are a key component of the immune system in mammals and are important molecular markers of adaptive genetic diversity in wild animal populations. Greater diversity of MHC genes within the population increases the chances of defence against pathogens and parasites, and thus the prospects for long-term survival. Golden jackal (Canis aureus) belongs to the family Canidae (dogs), suborder Caniformia, order Carnivora (carnivores). So far there are no published studies of MHC gene diversity on this species. The aim of this graduate thesis is to determine the level of diversity of the MHC system class II DQA, DQB and DRB genes in golden jackals from Israel. Three alleles at DQA locus, five alleles at DQB locus and four alleles at the DRB locus were found in 30 samples of jackal's ear. Methods used were DNA isolation, PCR method, electrophoresis, sequencing and molecular cloning. We found one new unique allele on DRB locus (DRB1*04802var). The rest of the alleles found are known from previous research on the other species of the dog family, which confirms the existance of a trans-species polymorphism. DQB and DRB loci show great diversity of alleles, large evolutionary distances, high ratio of non-synonymous versus synonymous substitutions, confirming the assumption that loci are influenced by positive selection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:603485
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 07:44:15