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master's thesis
Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:187929
Pehar, Sanja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Pehar, S. (2015). Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:187929

Pehar, Sanja. "Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:187929

Pehar, Sanja. "Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:187929

Pehar, S. (2015). 'Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:187929

Pehar S. Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:187929

S. Pehar, "Polimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:187929

Metadata
TitlePolimorfizam gena za interleukin 28B u osoba zaraženih virusom ljudske imunodeficijencije
Title (english)Polymorphism of interleukin 28B gene in human immunodeficiency virus infected individuals
AuthorSanja Pehar
MentorSnježana Židovec Lepej (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-04-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Zaraza virusom ljudske imunodeficijencije (HIV) povezuje se s progresivnom deficijencijom imunološkog sustava. CD4+ T-limfociti su osnovna subpopulacija stanica koju virus ljudske imunodeficijencije zaražava te tijekom infekcije dolazi do deplecije ovih stanica u krvi HIV-pozitivnih osoba što služi kao prognostički parametar za progresiju HIV-bolesti. Interferoni λ inhibiraju replikaciju različitih virusa. Polimorfizam jednog nukleotida rs12979860 u promotoru gena za interleukin-28B (jedan od interferona λ) utječe na koncentraciju tog citokina u organizmu. Osobe koje su C homozigoti imaju veće koncentracije IL-28B te sporiju progresiju bolesti. Cilj ovog istraživanja je odrediti povezanost između polimorfizma gena za IL-28B i imunološkog statusa HIV-om zaraženih osoba. Ispitanike sam podijelila u tri skupine prema apsolutnom broju CD4+ T-limfocita te metodom PCR-a u stvarnom vremenu odredila polimorfizam gena za IL-28B. Prva skupina su osobe koje su pri ulasku u kliničku skrb, te minimalno godinu dana nakon toga imale > 500 CD4+ limfocita / μl krvi. Druga skupina su osobe koje su u kliničku skrb ušle s < 200 CD4+ limfocita / μl krvi, ali je broj CD4+ limfocita porastao do > 500 CD4+ limfocita / μl krvi nakon početka terapije antiretrovirusnim lijekovima. Treća skupina su osobe koje su pri ulasku u kliničku skrb, te nakon minimalno godinu od započinjanja antiretroviralne terapije imale < 200 CD4+ limfocita / μl krvi. C homozigoti su najčešća skupina u prvoj i trećoj skupini ali i u čitavoj populaciji (50,5 %). Heterozigoti su prisutni u populaciji sa 36,7 % i najčešći je genotip u drugoj skupini ispitanika. T homozigoti su prisutni u populaciji sa 12,8 %, i najrjeđi je genotip u sve tri skupine. Nema statistički značajnih razlika između pojedinih skupina ispitanika. Istraživanje je pokazalo da nema povezanosti između progresije bolesti i IL-28 genotipa u HIV zaraženih osoba.
Abstract (english)
Infection with the human immunodeficiency virus (HIV) is associated with progressive deficiency of the immune system. CD4+ T-lymphocytes are the primary targets of HIV which leads to depletion of this cell population in HIV-infected individuals which serves as a prognostic marker for HIV disease progression. Interferons λ inhibt the replication of different viruses. Single-nucleotide polymorphism (SNP) of interleukin 28B (IL-28B, one of λ interferons) affects the concentration of that interleukin in the organism. C homozygotes have higher concentrations of that interleukin and therefore slower disease progression. The aim of this study is to determine the corellation between IL-28B SNP and immunological status of HIV-infected individuals. I have divided the patients into three groups considering their CD4+ count and using a real-time PCR test determined their IL-28B genotype. First group consisted of patients who had > 500 CD4+ lymphocytes / μl at entrance to care and at least for one year after. In the second group were patients who had < 200 CD4+ lymphocytes / μl but then thier CD4+ count rose to > 500 lymphocytes / μl and stayed as high for at least a year. Third group had patients who entered clinical care with < 200 CD4+ lymphocytes / μl at and remained so for at least a year. C homozygotes are most frequent in entire study group (50,5 %) as well as in the first and third group of patients. Heterozygotes were present in 36,7 % in the entire study group and were the most frequent in second group of patients. T homozygotes were the least frequent in an entire study population and all three groups. There were no statistically significant differences in genotype distribution between groups so this study showed that there is no corelation between disease progression and IL-28B genotype in HIV-infected individuals.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:187929
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 08:01:34