prikaz prve stranice dokumenta Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije
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master's thesis
Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:998848
Polak, Bruno
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Polak, B. (2015). Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:998848

Polak, Bruno. "Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:998848

Polak, Bruno. "Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:998848

Polak, B. (2015). 'Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:998848

Polak B. Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:998848

B. Polak, "Analiza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:998848

Metadata
TitleAnaliza mehanike metafaznog diobenog vretena u stanicama HeLa primjenom laserske mikrodisekcije
Title (english)Analysis of metaphase spindle mechanics in HeLa cells by laser microsurgery
AuthorBruno Polak
MentorIva Tolić (mentor)
MentorMaja Matulić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-07-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Glavna struktura koja upravlja procesom je diobeno vreteno, a jedna od klasa mikrotubula u toj strukturi su kinetohorna vlakna koja vežu kromosome putem kinetohora na centromeri. Trenutne spoznaje govore da dva sestrinska kinetohorna vlakna nisu u međusobnoj izravnoj interakciji. U ovom radu pažnja je usmjerena na opisivanje nove klase mikrotubula u vretenu a nazvana je premošćujući mikrotubuli. Ta nova klasa tvori vlakna antiparalelnih mikrotubula i lateralno povezuje sestrinska kinetohorna vlakna te sudjeluje u mehanici diobenog vretena. U provedenom istraživanju korišten je konfokalni mikroskop za snimanje živih stanica HeLa te je korištena laserska mikrodisekcija vanjskog kinetohornog vlakna, da bi se pokazala prisutnost premošćujućih mikrotubula te ispitala njihova uloga u distribuciji sila u vretenu. Ove su rezultate potvrdili eksperimenti sa stanicama sa smanjenom razinom proteina PRC1 koji je sastavni dio metafaznog vretena. Procesi u regiji između sestrinskih kromosoma analizirani su u stanicama s obilježenim proteinom EB3, uključenim u rast mikrotubula na + kraju. U oba eksperimenta potvrđena je prisutnost kompresivne sile i sile tenzije u metafaznom diobenom vretenu.
Abstract (english)
Main structure that orchestrates mitosis is the mitotic spindle, and one of the microtubule classes comprised within are the kinetochore fibers, which bind to chromosomes via kinetochores on the centromere. It is currently believed that there is no direct interaction between two sister kinetochore fibers. The focus of this research is to describe a novel class of microtubules in the spindle, which was named bridging microtubules. This new class forms antiparallel bundles, laterally connects sister kinetochore fibers and contributes to spindle mechanics. In conducted research, confocal microscope was used for live-cell imaging of HeLa, and the laser microsurgery was applied on outermost kinetochore fiber in order to confirm bridging microtubule presence and to test their function in force distribution in the spindle. By using cells with decreased level of PRC1 protein, protein involved in metaphase spindle mechanics, we showed that bridging bundle contributes to force balance in the spindle. Microtubule dynamics in the region between sister kinetochores was also analyzed in cells with labeled EB3 protein involved in the growth of microtubule 's + end. Laser microsurgery confirmed compressive and tensile force in the metaphase spindle in both experiments.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:998848
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 08:15:57