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master's thesis
Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:948848
Krsnik, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Krsnik, J. (2019). Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:948848

Krsnik, Jelena. "Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:948848

Krsnik, Jelena. "Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:948848

Krsnik, J. (2019). 'Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)', Master's thesis, University of Zagreb, Faculty of Science, accessed 16 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:948848

Krsnik J. Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 May 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:948848

J. Krsnik, "Utjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:948848

Metadata
TitleUtjecaj antidepresiva i amfetamina na fiziološke pokazatelje u invazivne strane vrste signalnog raka, Pacifastacus leniusculus (Dana, 1852)
Title (english)Effects of antidepressants and amphetamine on physiological parameters of the invasive alien species signal crayfish, Pacifastacus leniusculus (Dana, 1852)
AuthorJelena Krsnik
MentorSandra Hudina (mentor)
MentorSofia Ana Blažević (mentor)
Committee memberSandra Hudina (predsjednik povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
Committee memberJasenka Sremac (član povjerenstva)
Committee memberMladen Maradin (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Farmaceutici u slatkovodnim ekosustavima mogu utjecati na ponašanje i fiziologiju vodenih organizama jer su dizajnirani da brzo djeluju u ljudskom tijelu i izlučuju se bez razgradnje, pa u vodu dospijevaju farmakološki aktivni. U ovom radu istražila sam utjecaj farmaceutika prisutnih u rijeci Savi (antidepresivi citalopram i nortriptilin i opojna droga amfetamin) na signalnog raka, najuspješniju invazivnu stranu vrstu raka u Europi. Rakove sam izlagala okolišno relevantnoj (niskoj) ili terapeutskoj (visokoj) koncentraciji citaloprama, nortriptilina ili amfetamina tijekom 7 dana. S obzirom da odabrani farmaceutici utječu na metabolizam serotonina koji kod rakova kontrolira otpuštanje hiperglikemijskog hormona, mjerila sam razinu serotonina u cerebralnim ganglijima i razinu glukoze u hemolimfi te malondialdehid kao marker oksidativnog stresa. Rezultate izmjerenih parametara izloženih jedinki usporedila sam s kontrolnima koje nisu bile izložene spojevima. Nortriptilin u visokoj koncentraciji povisio je razinu serotonina u cerebralnim ganglijima u odnosu na grupu izloženu niskoj koncentraciji. Kod rakova izloženih niskoj koncentraciji nortriptilina zabilježeno je povišenje razine glukoze u hemolimfi u odnosu na kontrolne grupe, a izlaganje amfetaminu u obje koncentracije snizilo je razine glukoze u hemolimfi. Nisam zabilježila povišenje markera oksidativnog stresa. U budućim istraživanjima potrebno je produljiti vrijeme izlaganja i istražiti utjecaj na ostale hormonima regulirane fiziološke parametre (rast i razmnožavanje).
Abstract (english)
Pharmaceutical pollution can affect behavior and physiology of freshwater organisms since pharmaceuticals act quickly in the human body, excrete without degrading and enter freshwaters still pharmacologically active. This study explores whether pharmaceuticals present in the Sava River (antidepressants Citalopram and Nortriptyline, illicit drug Amphetamine) have an effect on the physiology of the most successful crustacean invader of European freshwaters, the Signal crayfish. I exposed individuals to environmentally relevant (low), or therapeutic (high) concentration of Citalopram, Nortriptyline or Amphetamine dissolved in ambient water for 7 days. Serotonin mediates the release of crustacean hyperglycemic hormone (cHH) that controls hemolymph glucose concentration, and since these chemicals alter serotogenic metabolism, I measured concentration of serotonin in the brain, concentration of glucose in hemolymph, and malondialdehyde, marker of oxidative stress, and compared the results of exposed individuals to that of control-unexposed groups. High concentration of Nortriptyline elevated serotonin levels in comparison to exposure to low concentrations. Glucose levels were higher than in control group at low concentration of Nortriptyline, and lower than in control group at both concentrations of Amphetamine. There was no influence on levels of oxidative stress biomarker. Future research should include long-term exposure and examine effects of pharmaceuticals on growth and reproduction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:948848
Study programmeTitle: Enviromental Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-09-26
Terms of useLicence In copyright icon
Created on2019-10-14 09:29:43