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master's thesis
Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:804377
Borić, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Borić, A. (2019). Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:804377

Borić, Ana. "Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:804377

Borić, Ana. "Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:804377

Borić, A. (2019). 'Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:804377

Borić A. Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:804377

A. Borić, "Ekspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:804377

Metadata
TitleEkspresija izoformi proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka
Title (english)The expression of p53 and p73 isoforms in human soft tissue sarcoma cell lines
AuthorAna Borić
MentorNeda Slade (mentor)
MentorMaja Matulić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberBožena Mitić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Sarkomi mekih tkiva su zloćudni tumori mezenhimalnog porijekla. Mehanizam tumorigeneze sarkoma mekih tkiva još uvijek je neobjašnjen. Mutacije u genu TP53 su najčešće genetske promjene u tumorima. Porodica p53 obuhvaća gene TP53, TP63 i TP73 koji reguliraju mnoge vitalne biološke procese, uključujući diferencijaciju stanica, proliferaciju i apoptozu. Svaki gen proizvodi proteinske izoforme kroz više mehanizama - alternativnim prekrajanjem, korištenjem alternativnih promotora te različitih mjesta početka translacije. Za razliku od p53, protein p73 prekomjerno je eksprimiran u različitim tumorima i rijetko mutiran. Cilj ovog diplomskog rada je odrediti gensku ekspresiju izoformi gena TP53 i TP73 te proteina p53 i p73 u staničnim linijama sarkoma mekih tkiva čovjeka. Rezultati su pokazali jaču gensku ekspresiju izoformi p53α i Δ133p53α, dok je analiza proteinske ekspresije pokazala višu razinu p53α i Δ40p53α u staničnim linijama u kojima je TP53 mutiran. U staničnim linijama u kojima TP53 nije bio mutiran detektirala sam slabu ekspresiju izoformi p53α, Δ133p53α i p53β. Izoforme gena TP73 slabo su eksprimirane u svim staničnim linijama, a jedino je protein TAp73α značajno eksprimiran u svim staničnim linijama.
Abstract (english)
Soft tissue sarcomas are malignant tumors of mesenchymal origin. The tumorigenic mechanism of soft tissue sarcoma is still unexplained. Mutations in the TP53 gene are the most common genetic changes in tumors. The p53 family includes the TP53, TP63 and TP73 genes that regulate many vital biological processes including cell differentiation, proliferation, and apoptosis. Each gene produces protein isoforms through multiple mechanisms – alternative splicing, use of alternative promoters and alternative translation initiation sites. Unlike p53, protein p73 is rarely mutated but is overexpressed in various tumors. The aim of this graduate thesis is to determine the expression profile of the TP53 and TP73 genes and p53 and p73 proteins in human soft tissue sarcoma cell lines. The results showed stronger gene expression of p53α and Δ133p53α isoforms, while protein analysis revealed higher levels of p53α and Δ40p53α proteins in cell lines with mutated TP53. In TP53 mutant cell lines, I detected poor expression of isoforms p53α, Δ133p53α and p53β. TP73 gene isoforms were poorly expressed in all cell lines, and only TAp73α protein was significantly expressed in all cell lines.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:804377
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-03-29
Terms of useLicence In copyright icon
Created on2019-10-16 07:53:57