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master's thesis
Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:329753
Vučenović, Dunja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Vučenović, D. (2015). Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:329753

Vučenović, Dunja. "Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:329753

Vučenović, Dunja. "Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:329753

Vučenović, D. (2015). 'Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:329753

Vučenović D. Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:329753

D. Vučenović, "Analiza N-glikoma imunoglobulina G u blizanaca s križoboljom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:329753

Metadata
TitleAnaliza N-glikoma imunoglobulina G u blizanaca s križoboljom
Title (english)Glycomic phenotyping of twin patients with low back pain
AuthorDunja Vučenović
MentorFrances Mary Kingsley Williams (mentor)
MentorVlatka Zoldoš (mentor)
Committee memberVlatka Zoldoš (predsjednik povjerenstva)
Committee memberVesna Benković (član povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
Committee memberKristian Vlahoviček (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Križobolja je sveprisutan mišićno-skeletni sindrom koji se javlja u svim dobnim skupinama. Detaljne informacije o dijagnozi ovog sindroma potrebne su zbog njegove kliničke i socijalne važnosti. Trenutno postoje ograničeni biomarkeri (uglavnom su to snimke dobivene magnetskom rezonancijom) zbog čega je potrebno razviti nove, jednostavnije metode rane detekcije križobolje. Jedan od glavnih uzroka kronične križobolje jest lokalizirana upala u epiduralnom prostoru. Kako je svaki upalni proces povezan s brojnim promjenama na mjestu upale, tako su uočene i promjene u glikanima vezanim za Fc regiju imunoglobulina G (IgG). U ovom radu nastojala sam odrediti postoje li različiti profili količine glikana kod blizanca iz TwinsUK baze podataka s križoboljom i zdravih pojedinaca. Da bih to ostvarila, analizirala sam N-glikom IgG-a 4416 blizanaca koji su odgovorili na upitnike o povijesti boli, čiji je glikanski profil određen i koji su podvrgnuti snimanju kralježnice magnetskom rezonancijom. S dobivenim podatcima sam, koristeći statističke metode za analizu blizanaca, tražila asocijaciju N-glikana s fenotipovima boli. Dodatno sam koristila i metode koje se koriste u genomici za određivanje razlike u ekspresiji gena kod pacijenata i kontrola te tako dobila module različitih količina N-glikana. Dobivenim modulima kreirala sam prediktivne algoritme koji govore o vjerojatnosti razvoja križobolje kod novih ispitanika.
Abstract (english)
Lumbago is a common musculoskeletal condition in all ages. Owing to its clinical and social impact, a clear diagnosis of this syndrome is needed in order to define besides pain intensity also the interference of pain with daily and work activity. Currently, there are limited biomarkers (mostly imaging), but there is a need for novel and simpler detection methods. One of the major determinants of pain in persistent lumbago is localised inflammation in epidural space. As every inflammatory process is linked with changes on the inflamed region, so are altered N-glycan IgG compositions. In this study, using classical twin design conducted between twins from TwinsUK registry, I assessed whether twins reporting episodes of lumbago had detectable levels of altered IgG glycosylation. I analysed IgG N-glycome of 4416 twins having completed pain history questionnaires, whose glycan profile was determined and who underwent magnetic-resonance spine scan. With this data, using statistical methods for twin studies, I looked for association between glycan quantities and pain phenotypes. Additionally, I used methods applied in genomics and obtained modules of glycans that show different patterns of glycan quantities. With these modules I built prediction algorithms that are giving likelihood of person reporting episodes of lumbago.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:329753
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-04-24 09:25:53