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doctoral thesis
Prisutnost virusa u populacijama šišmiša u Hrvatskoj
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:093402
Šimić, Ivana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Šimić, I. (2019). Prisutnost virusa u populacijama šišmiša u Hrvatskoj (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:093402

Šimić, Ivana. "Prisutnost virusa u populacijama šišmiša u Hrvatskoj." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:093402

Šimić, Ivana. "Prisutnost virusa u populacijama šišmiša u Hrvatskoj." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:093402

Šimić, I. (2019). 'Prisutnost virusa u populacijama šišmiša u Hrvatskoj', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:093402

Šimić I. Prisutnost virusa u populacijama šišmiša u Hrvatskoj [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:093402

I. Šimić, "Prisutnost virusa u populacijama šišmiša u Hrvatskoj", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:093402

Metadata
TitlePrisutnost virusa u populacijama šišmiša u Hrvatskoj
Title (english)The presence of viruses in bats populations in Croatia
AuthorIvana Šimić
MentorTomislav Bedeković (mentor)
MentorKrešo Bendelja (komentor)
Committee memberSilvija Černi (predsjednik povjerenstva)
Committee memberSnježana Židovec-Lepej (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Šišmiši su prepoznati kao rezervoari raznih virusa, uključujući i one zoonotskog potencijala diljem svijeta. U Hrvatskoj dosad nije bilo sustavnog pretraživanja virusa šišmiša te je cilj ovog istraživanja bio razotkriti virom šišmiša i utvrditi njihov javnozdravstveni značaj. U ovom istraživanju utvrđivana je prisutnost specifičnih protutijela za virus bjesnoće u krvi šišmiša virus neutralizacijskim testom te je određivana prisutnost genoma lyssavirusa u obriscima sluznice usne šupljine lančanom reakcijom polimerazom. Utvrđivana je i prisutnost drugih virusnih porodica u obriscima usne šupljine, fecesu i gvanu sekvenciranjem nove generacije. Iako genom lyssavirusa nije utvrđen u obriscima usne šupljine, po prvi puta je u Hrvatskoj utvrđena seroprevalencija specifičnih protutijela za virus EBLV-1 od 5,71% čime je utvrđeno da šišmiši predstavljaju malu, ali ne i zanemarivu javnozdravstvenu prijetnju. Iako su sekvenciranjem nove generacije pronađene 63 virusne porodice, utvrđeni su i genomi adeno-associated virusa, ambidensovirusa, iflavirusa i circo-like virusa, međutim nisu otkriveni drugi zoonotski patogeni.
Abstract (english)
Bats are recognized as reservoirs of many viruses with special emphasis on those with zoonotic potential. There was no systematic research of bats' viruses in Croatia so the aim of the present study was to reveal the virome of bats and their public health relevance. In this research specific antilyssaviral antibodies were detected in blood by virus neutralization test and the presence of lyssavirus genome was investigated using polymerase chain reaction by testing bats oral swabs. Furthermore, the presence of other virus families was investigated by next generation sequencing of oral swab samples, feces and guano. Although the presence of lyssavirus genom was not recorded, for the first time in Croatia antilyssaviral antibodies were detected in 5.7% of tested animals, suggesting that bats present small but not negligible threat to public health. Furthermore, 63 viral families were detected by next generation sequencing, with genomes of adeno-associated virus, ambidensovirus, iflavirus and circo-like virus, however, no other zoonotic pathogens were found.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:093402
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-10-22 08:43:04