prikaz prve stranice dokumenta Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima
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doctoral thesis
Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:650841
Krasnići, Nesrete
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Krasnići, N. (2019). Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:650841

Krasnići, Nesrete. "Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:650841

Krasnići, Nesrete. "Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima." Doctoral thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:650841

Krasnići, N. (2019). 'Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:650841

Krasnići N. Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:650841

N. Krasnići, "Raspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:650841

Metadata
TitleRaspodjela odabranih metala među citosolskim biomolekulama škrga i jetre klenova (Squalius cephalus (Linnaeus) i Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) iz rijeka onečišćenih metalima
Title (english)Distribution of selected metals among cytosolic biomolecules in gills and liver of chub (Squalius cephalus (Linnaeus) and Squalius vardarensis Karaman; Actinopterygii, Cyprinidae) from metal contaminated natural waters
AuthorNesrete Krasnići
MentorZrinka Dragun (mentor)
Committee memberDušica Ivanković (predsjednik povjerenstva)
Committee memberSaša Kazazić (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-10-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Citosolske raspodjele nekoliko odabranih elemenata (Cd, Co, Cu, Fe, Mn, Mo, Pb, Se i Zn) u jetrima i ńkrgama dvaju slatkovodnih bioindikatorskih organizama, klena (Squalius cephalus) iz rijeke Sutle u Hrvatskoj i vardarskog klena (Squalius vardarensis) iz triju rijeka sjeveroistoĉne Sjeverne Makedonije, izuĉavane su primjenom tekućinske kromatografije visoke djelotvornosti s iskljuĉenjem po veliĉini (SEC-HPLC) i spektrometrije masa visoke rezolucije s induktivno spregnutom plazmom (HR ICP-MS). OdreĊene su raspodjele metala/nemetala meĊu citosolskim biomolekulama razliĉitih molekulskih masa svojstvene uvjetima niske izloņenosti metalima u rijeĉnoj vodi, kao i promjene u raspodjelama koje su javljaju kao posljedica povińenih razina izloņenosti. Primjena anionsko izmjenjivaĉke kromatografije (AEC-HPLC) kao drugog stupnja razdvajanja i proĉińćavanja citosolskih biomolekula te dviju tehnika spektrometrije masa (MALDI-TOF-MS i LC-MS/MS) omogućila je identifikaciju ili precizno odreĊivanje molekulskih masa nekoliko citosolskih biomolekula koje veņu metale u jetrima i ńkrgama vardarskog klena, poput izoformi metalotioneina koje veņu Cd, Cu i Zn, hemoglobinskih podjedinica koje veņu Fe te toplinski stabilnih biomolekula koje veņu Mo. Primijenjeni analitiĉki pristup pokazao se korisnim u istraņivanju unutarstaniĉne sudbine i ponańanja metala u organima riba te otkrivanju potencijalnih novih biomarkera izloņenosti i uĉinaka metala.
Abstract (english)
The cytosolic distributions of several selected elements (Cd, Co, Cu, Fe, Mn, Mo, Pb, Se, and Zn) in the liver and gills of two freshwater bioindicators, European chub (Squalius cephalus) from the Sutla River in Croatia and Vardar chub (Squalius vardarensis) from three rivers of north-eastern Macedonia, were studied using size-exclusion high performance liquid chromatography (SEC-HPLC) and high resolution inductively coupled plasma mass spectrometry (HR ICP-MS). Distributions of metals/non-metal among the biomolecules of different molecular masses which are characteristic for low metal exposure in the river water were determined, as well as the changes in distribution which occur as a consequence of increased exposure to metals. The use of anion-exchange high performance liquid chromatography (AEC-HPLC) as the second step of separation and purification of cytosolic biomolecules, as well as the use of two mass spectrometry techniques (MALDI-TOF-MS and LC-MS/MS) have enabled the identification or exact determination of molecular masses of several cytosolic biomolecules that bind metals in the liver and gills of Vardar chub, such as the metallothionein isoforms that bind Cd, Cu and Zn, the hemoglobin subunits that bind Fe and heat stable biomolecules that bind Mo. Applied analytical approach has proven to be useful in the study of intracellular fate and behavior of metals in organs of fish, as well as in the process of discovery of potential new biomarkers of metal exposure and effects.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:650841
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-10-22 09:14:34