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undergraduate thesis
Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:926184
Radman, Katarina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Radman, K. (2019). Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:926184

Radman, Katarina. "Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima." Undergraduate thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:926184

Radman, Katarina. "Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima." Undergraduate thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:926184

Radman, K. (2019). 'Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:926184

Radman K. Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:926184

K. Radman, "Evolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:926184

Metadata
TitleEvolucijske zavrzlame RNaze P: od katalitičke RNA do isključivo proteinskog enzima
Title (english)Evolutionary conundrum of RNase P: from a catalytic RNA form to a protein only enzyme form
AuthorKatarina Radman
MentorIta Gruić-Sovulj (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
RNaza P je esencijalna endonukleaza koja katalizira procesiranje 5'-vodećeg slijeda pre-tRNA. Postoje dva tipa RNaze P: RNA-ovisni enzim tj ribonukleoprotein te isključivo proteinska RNaza P (PRORP). Oba oblika koriste sličan mehanizam katalize i prepoznavanja supstrata te je nedavno otkriveno da isključivo proteinski oblik postoji u svim domenama života, a ne samo kod eukariota, što je dovelo do pitanja zašto su oba oblika RNaze P opstala i koji je oblik onda zapravo ancestralni. Istraživanja komponenti ribonukleoproteinskog oblika RNaze P otkrila su da osim što ovaj enzim ima ulogu katalize procesiranje pre-tRNA, njegove podjedinice sudjeluju u mnogim drugim biokemijskim procesima koji su bitni za normalno funkcioniranje stanice zbog čega je njegov opstanak u stanici esencijalan. RNP oblici RNaze P u svim domenama života imaju visoko očuvano aktivno mjesto katalize, za razliku od PRORP oblika koji ne dijele nikakvu sličnost. Ove činjenice ukazuju na to da je RNP oblik ancestralni, a da su PRORP oblici kasnije neovisno konvergentno evoluirali. Evolucijska priča RNaze P veoma je kompleksna i postoje mnoga nagađanja kako se odvijala, no pravi odgovor još uvijek ostaje enigma
Abstract (english)
RNase P is an essential endonuclease responsible for catalyzing 5’ end maturation of pre-tRNAs. Two types of RNase P enzymes exist: RNA-dependent enzymes (ribonucleoproteins) and protein-only RNase Ps (PRORP). A recent discovery that both types of RNase P exist in all domains of life and that both types use a similar mechanism for catalysis and substrate recognition, inspires questions relating to the ancestral form of RNase P as well as to the reasoning for retention of both types in contemporary organisms. The protein components of RNA-dependent enzymes are not only involved in the catalyzes of 5' end maturation but they also play an important role in various biochemical processes in the cell. This may provide a plausible explanation why the retention of these proteins is essential. The active site of the RNA-dependent type of RNase P is universally conserved in all domains of life, unlike the active site of the protein only types of RNase P which greatly varies. This suggests that the RNA-dependent type of RNase P is the ancestral form and that the diverse PRORP forms convergently evolved afterwards. The evolutionary story of RNase P is very complex and there are many speculations on how RNase P evolved but the real answer is still not clear.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:217:926184
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-10-25 07:20:01