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master's thesis
Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:754464
Grba, Adriana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Grba, A. (2014). Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:754464

Grba, Adriana. "Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:754464

Grba, Adriana. "Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:754464

Grba, A. (2014). 'Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:754464

Grba A. Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:754464

A. Grba, "Uloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:754464

Metadata
TitleUloga alela gena HLA-DPB1 u ishodu transplantacije krvotvornih matičnih stanica s nesrodnog davatelja
Title (english)The role of HLA-DPB1 alleles on outcome of hematopoietic stem cell transplantation from unrelated donor
AuthorAdriana Grba
MentorZorana Grubić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberZlatko Liber (član povjerenstva)
Committee memberMilorad Mrakovčić (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U ovom radu analizirali smo podudarnost HLA među parovima primatelj-nesrodni davatelj u programu nesrodne transplantacije krvotvornih matičnih stanica (TKMS). Unutar ispitivane skupine odredili smo raspodjelu alela HLA-DPB1 među bolesnicima, njihovim nesrodnim davateljima i kontrolama, broj nepodudarnosti na lokusima HLA-A, -B, -C, -DRB1, -DQB1 i –DPB1 između primatelja i nesrodnog davatelja te ispitali povezanost dopuštenih i nedopuštenih nepodudarnosti HLA-DPB1 s ishodom TKMS. Skupina je obuhvatila 57 parova primatelj-davatelj kojima su određeni aleli HLA-A, -B, -C, -DRB1, -DQB1 i –DPB1 metodom lančane reakcije polimerazom i specifičnim početnicama ili probama za alele HLA (metode: PCR-SSP i PCR-SSO). Među bolesnicima najčešći alel bio je HLA-DPB1*04:01 (42,1%) što je u skladu s rezultatima za kontrolnu skupinu. Unutar cjelokupne skupine bolesnika nije otkrivena statistički značajna razlika u preživljavanju između bolesnika s 0, 1 ili 2 nepodudarnosti DPB1. Statistički značajno bolje preživljavanje bolesnika s 2 nepodudarnosti za alele DPB1 uočeno je samo u skupini bolesnika koji su imali davatelja 10/10. Nije uočena statistički značajna razlika u preživljavanju između bolesnika s dopuštenim i onih s nedopuštenim nepodudarnostima DPB1. Za donošenje konačnog zaključka o nepodudarnosti za alele HLA-DPB1 u ishodu TKMS neophodno je provesti veći broj istraživanja.
Abstract (english)
In the present study the correlation between HLA mismatches among recipient-unrelated donor in hematopoietic stem cell transplantation (HSCT) were analyzed. Tested group comprised of 57 pairs. The distribution of DPB1 alleles in the group of patients, unrelated donors and healthy controls was compared. The next goal was to analyze the number of mismatches at HLA-A,-B,-C, -DRB1, -DQB1 and -DPB1 loci between recipient-donor pairs and their correlation on the outcome of HSCT. HLA alleles were tested by Polymerase Chain Reaction and specific primers or probes (PCR-SSP and PCR-SSO methods). Among all three tested groups DPB1*04:01 allele was the most frequent (42.1%). No statistically significant difference in survival after HSCT was observed between patients with 0, 1, or 2 mismatches at DPB1 locus. In the group of 10/10 matched pairs significantly better survival was observed for patients with 2 DPB1 mismatches. There was no statistically significant difference in survival between patients with permissive and non-permissive DPB1 mismatches. For the final conclusion about the necessity of matching for HLA-DPB1 alleles it is required to enlarge the number of studies.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:754464
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 11:03:22