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master's thesis
Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.)
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:578998
Milošić, Anita
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Milošić, A. (2014). Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:578998

Milošić, Anita. "Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.)." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:578998

Milošić, Anita. "Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.)." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:578998

Milošić, A. (2014). 'Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.)', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:578998

Milošić A. Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:578998

A. Milošić, "Utjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:578998

Metadata
TitleUtjecaj čestica nanosrebra na rast i pojavu oksidacijskog stresa u korjenčićima luka (Allium cepa L.)
Title (english)Influence of silver nanoparticles on growth and appearance of oxidative stress in Allium cepa L. roots
AuthorAnita Milošić
MentorBiljana Balen (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-07-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nanočestice su trenutno vrlo živo područje istraživanja zahvaljujući svojim jedinstvenim električnim, kemijskim i fizičkim svojstvima, a zbog sve češće tržišne uporabe postavlja se pitanje koliko su štetne za žive organizme i okoliš. U ovom radu istražen je utjecaj nanočestica srebra (AgNP) na rast i pojavu oksidacijskog stresa u korjenčićima luka. Uspoređena je toksičnost primijenjenih koncentracija (25, 50, 75, 100 i 500 μM) AgNP s učinkom ionskog srebra (AgNO3) istih koncentracija. Također je uspoređen učinak nanosrebra stabiliziranog s tri različita organska omotača (AgNP-citrat, AgNP-PVP i AgNP-CTAB). Rezultati su pokazali da je ionsko srebro pri svim ispitanim koncentracijama jače inhibiralo rast korjenčića luka od nanosrebra. Sadržaj malondialdehida (MDA), kao pokazatelja oksidacijskog stresa, bio je povećan nakon tretmana ionskim srebrom i nanosrebrom AgNP-CTAB, a smanjen nakon tretmana nanosrebrom AgNP-citrat i AgNP-PVP. Sadržaj proteinskih karbonila bio je smanjen nakon tretmana ionskim srebrom, dok je samo nanosrebro AgNP-citrat pokazalo smanjenje sadržaja karbonila kako su rasle ispitane koncentracije. Tretmani nanosrebrom AgNP-PVP i AgNP-CTAB nisu doveli do promjene u sadržaju karbonila u odnosu na kontrolu. Sva tri ispitana antioksidacijska enzima (katalaza, pirogalol peroksidaza i askorbat peroksidaza) pokazala su promjene u aktivnosti, koje su posljedica izlaganja korjenčića ionskom srebru i nanosrebru.
Abstract (english)
Nanoparticles became the subject of interest in science thanks to their unique electric, chemical and physical properties. Since they are commonly used in consumer products, there is a need to investigate their possible negative effect on living organisms and environment. The present study investigated the effect of silver nanoparticles (AgNP) on growth and appearance of oxidative stress in A. cepa roots. The toxicity of AgNPs and AgNO3 applied at the same concentrations (25, 50, 75, 100 and 500 μM) was also compared. The effect of three different organic coatings, AgNP-citrate, AgNP-PVP and AgNP-CTAB, was additionally analysed. The results showed that ionic silver inhibited root growth more strongly than AgNP. Malondialdehyde (MDA) content, as a parameter of oxidative stress, was increased after treatment with ionic silver and nanosilver AgNP-CTAB and decreased after treatment with nanosilver AgNP-citrate and AgNP-PVP. Protein carbonyl content was also decreased after treatment with ionic silver and AgNP-citrate, and no changes were observed after AgNP-PVP and AgNP-CTAB treatment. All of three investigated antioxidant enzymes (CAT, PPX, APX) showed changes in activity that could be a consequence of root exposure to ionic and nanosilver.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:578998
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 11:43:24