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master's thesis
Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:679665
Lambert, Ines
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Lambert, I. (2019). Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff. (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:679665

Lambert, Ines. "Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:679665

Lambert, Ines. "Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:679665

Lambert, I. (2019). 'Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:679665

Lambert I. Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:679665

I. Lambert, "Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:679665

Metadata
TitleUtjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.
Title (english)Influence of salinity and osmotic stress on protein glycosylation in Mammillaria gracilis Pffeif.
AuthorInes Lambert
MentorBiljana Balen (mentor)
MentorPetra Cvjetko (mentor)
Committee memberSandra Radić Brkanac (predsjednik povjerenstva)
Committee memberDamir Sirovina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U ovom su radu uspoređivani utjecaji solnog i osmotskog stresa na proteine izolirane iz kalusnog i tumorskog tkiva kaktusa vrste Mamillaria gracilis Pfeiff. uzgajanih u uvjetima in vitro. Budući da se radi o biljci sa CAM metabolizmom, koja je prilagođena na sušne uvjete staništa i osjetljiva na promjene u okolišnim čimbenicima, ona je prikladan modelni organizam za proučavanje mehanizama u biljnim stanicama koji se aktiviraju tijekom izlaganja biljaka solnom i osmotskom stresu. U ovom je istraživanju pokazano kako izlaganje tkiva vrste M. gracilis soli i manitolu mijenja ekspresiju ukupnih topivih staničnih proteina i izaziva promjene u njihovoj N-glikozilaciji, važnoj posttranslacijskoj modifikaciji proteina. Osmotski stres izazvan manitolom imao je jači utjecaj na N-glikozilaciju proteina kalusa, dok je na tumorsko tkivo veći utjecaj imao solni stres (NaCl). S obzirom da je uočena razlika u ekspresiji između glikoproteina kalusnog i tumorskog tkiva nakon izloženosti istom tipu abiotičkog stresa, može se zaključiti da je N-glikozilacija proteina specifična za određeni tip tkiva. S druge strane, razlika u ekspresiji glikoproteina unutar istog tipa tkiva, nakon izloženosti različitim vrstama stresora u podlozi (NaCl ili manitol), navodi na zaključak da stupanj glikozilacije ovisi o vrsti stresa kojoj je tkivo izloženo.
Abstract (english)
In this paper, the effects of salt and osmotic stress on proteins isolated from callus and tumour tissue lines of Mamillaria gracilis Pfeiff. cacti, grown in vitro, were compared. Because it is a plant with CAM metabolism adapted to arid habitats and sensitive to changes in environmental factors, it is a suitable model organism to study the mechanisms in plant cells that are activated during plant exposure to salt and osmotic stress. In this study, it was shown that the exposure of M. gracilis tissues to salt and mannitol alters the expression of total soluble cellular proteins and induces changes in protein N-glycosylation, a very important posttranslational modification. Osmotic stress induced by mannitol had stronger impact on callus protein N-glycosylation, while tumour tissue was found to be more sensitive to salt stress (NaCl). Difference in glycoprotein expression, which was found between callus and tumour tissue after their exposure to the same type of abiotic stress, brings to conclusion that N-glycosylation is specific to certain tissues type. On the other hand, difference in glycoprotein expression, detected within the same tissue type after exposure to different stressors (NaCl or mannitol), implies that level of protein N-glycosylation is dependent on the type of the stressor.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:679665
Study programmeTitle: Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra edukacije biologije (magistar/magistra edukacije biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-20 08:01:37