prikaz prve stranice dokumenta Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma
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master's thesis
Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:619027
Sasi, Biljana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Sasi, B. (2015). Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:619027

Sasi, Biljana. "Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:619027

Sasi, Biljana. "Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:619027

Sasi, B. (2015). 'Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:619027

Sasi B. Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:619027

B. Sasi, "Razlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:619027

Metadata
TitleRazlike u replikaciji DNA između podskupina difuznog B-velikostaničnog limfoma
Title (english)DNA replication differences between diffuse large B-cell lymphoma subtypes
AuthorBiljana Sasi
MentorPetra Korać (mentor)
Committee memberPetra Korać (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
Committee memberVlatka Zoldoš (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Difuzni B-velikostanični limfom (DLBCL) je neoplazma B-limfocita koja pokazuje karakterističan difuzni uzorak u zahvaćenom tkivu. Čini oko 30% slučajeva ne-Hodgkinskog limfoma u odraslih. Na temelju profila genske ekspresije, DLBCL je moguće podijeliti u podskupine GCB (skupina B-stanica koja se nalazi u germinativnom centru folikula limfnih čvorova) i ne-GCB (skupina B-stanica koja se ne nalazi u germinativnom centru folikula limfnih čvorova). Kod velikog broja slučajeva s DLBCL-om mogu se detektirati brojni genski rearanžmani, od kojih je najčešća translokacija gena BCL6, gena BCL2, te gena c-MYC. Cilj ovog rada je analiza promjena u količini transkripata gena uključenih u proces replikacije i ponovne uspostave kromatina kod podskupina DLBCL-a. Takve promjene imaju ulogu u smanjenoj preciznosti odvijanja replikacije što dovodi do nakupljanja velikog broja pogrešaka u tumorskim stanicama, te potencijalno do progresije tumora. Uz pomoć imunohistokemijskog algoritma prema Hans i sur., svi slučajevi DLBCL-a podijeljeni su u GCB i ne-GCB podskupinu. RNA je izolirana iz svih uzoraka i reverznom transkripcijom prevedena u cDNA. q-RT PCR analiza ekspresije gena DNMT1, PCNA, MCM2, MCM7, GMNN, CDT1, EZH2 i p300 pokazala je povećanu ekspresiju gena CDT1 i DNMT1 i smanjenu ekspresiju gena PCNA, MCM2, MCM7 i EZH2 u GCB podskupini u odnosu na ne-GCB podskupinu. Rezultati ukazuju na potencijalan doprinos promjena ekspresije CDT1, DNMT1 i MCM7 patogenezi DLBCL-a.
Abstract (english)
Diffuse large B-cell lymphoma (DLBCL) is a neoplasm of B-cells with a characteristic diffuse pattern of distribution. It accounts for approximately 30% of non-Hodgkin lymphomas in elderly. DLBCL can be stratified, based on gene expression profiling, into GCB (germinal centre B-cell like) and non-GCB (non-germinal centre B-cell like) subtypes. In most DLBCL cases, a large number of genetic rearrangements can be detected. The most frequent is BCL6 translocation, followed by translocations of BCL2 and c-MYC. The aim of this study was to analyse changes in the amount of proteins involved in the replication process and the chromatin reassembly mechanisms in DLBCL subtypes. These changes might be responsible for aberrations in replication accuracy which further leads to accumulation of additional mutations in tumor cells causing tumor progression. DLBCL cases were classified into GCB and non-GCB subtyes using immunohistochemical algorithm by Hans et al. RNA from all samples was extracted and converted to cDNA by reverse transcription. q-RT PCR analysis of DNMT1, PCNA, MCM2, MCM7, GMNN, CDT1, EZH2 and p300 expression showed an increased expression of CDT1 and DNMT1 as well as a decreased expression of PCNA, MCM2, MCM7 and EZH2 in GCB when compared with a non-GCB subtype. Results suggest contribution of replication and chromatin reassembly machinery components to DLBCL pathogenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:619027
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-25 08:24:26