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master's thesis
Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra
Zagreb: University of Zagreb, Faculty of Science, 2015. urn:nbn:hr:217:207801
Vičić, Luna
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Vičić, L. (2015). Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:207801

Vičić, Luna. "Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:207801

Vičić, Luna. "Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra." Master's thesis, University of Zagreb, Faculty of Science, 2015. https://urn.nsk.hr/urn:nbn:hr:217:207801

Vičić, L. (2015). 'Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:207801

Vičić L. Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2015 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:207801

L. Vičić, "Učinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:217:207801

Metadata
TitleUčinak kvercetina na preživljenje neurona P19 u prisutnosti toksičnih koncentracija iona bakra
Title (english)Effect of quercetin on viability of P19 neurons in the presence of toxic concentrations of copper ions
AuthorLuna Vičić
MentorMaja Jazvinšćak Jembrek (mentor)
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberSven Jelaska (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2015-02-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Oksidacijski stres je jedan od ključnih čimbenika u nastanku i progresiji brojnih neurodegenerativnih bolesti. Cilj istraživanja bio je utvrditi učinak flavonoida kvercetina, jednog od najpotentnijih antioksidansa, na preživljenje neurona P19 u uvjetima oksidacijskog stresa izazvanog suviškom iona bakra. Kod umjereno oštećenih neurona obrađenih s 0,5 mM CuSO4 tijekom 24 sata, kvercetin u koncentraciji od 150 μM je povećao preživljenje, te smanjio bakrom izazvan porast stvaranja reaktivnih kisikovih vrsta (RKV) i aktivnost kaspaza-3/7. U istim je uvjetima kvercetin dodatno smanjio bakrom izazvan pad u razini staničnog glutationa. Učinak kvercetina na preživljenje neurona P19 spriječen je u prisutnosti vortmanina, inhibitora PI3K/Akt signalnog puta. Obrada s 1 mM CuSO4 uz dodatni pad preživljenja, izazvala je i izrazit porast RKV i aktivaciju kaspaza-3/7, kao i smanjenje ekspresije antiapoptotskog gena Bcl-2 te porast ekspresije proapoptotskog gena Bax na razini mRNA, ali se toksični učinak bakra nije smanjio u prisutnosti 150 μM kvercetina. Dobiveni rezultati upućuju na neuroprotektivno djelovanje kvercetina u uvjetima umjerenog oksidacijskog oštećenja izazvanog ionima bakra koje se temelji na antioksidacijskom i antiapoptotskom djelovanju, kao i modulaciji PI3K/Akt signalnog puta.
Abstract (english)
Oxidative stress is one of the key factors in the development and progression of many neurodegenerative diseases. The aim of the present study was to explore the effects of the flavonoid quercetin, one of the most potent antioxidants, on the survival of P19 neurons under conditions of oxidative stress provoked by excess copper ions. In moderately injured neurons exposed to 0.5 mM CuSO4 for 24 hours, 150 μM quercetin increased viability and reduced copper-induced increase in the production of reactive oxygen species (ROS) and the caspase-3/7 activity. Quercetin also decreased copper-induced depletion of glutathione content. Effect of quercetin on survival of P19 neurons was prevented in the presence of wortmannin, an inhibitor of the PI3K/Akt signaling pathway. Treatment with 1 mM CuSO4 induced further decrease in neuronal survival, together with very pronounced ROS accumulation and activation of caspase-3/7, as well as decreased expression of antiapoptotic gene Bcl-2 and increased expression of proapototic gene Bax at the transcriptional level, but the toxic effect of copper was not reduced in the presence of 150 μM quercetin. The obtained results suggest that neuroprotective effect of quercetin against moderate oxidative injury induced by excess copper ions are related to its antioxidative and antiapoptotic activity, as well as modulation of the PI3K/Akt signaling pathway.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:207801
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-25 08:35:37