prikaz prve stranice dokumenta Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša
  Download
PDF 1.31 MB
master's thesis
Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:685671
Bašić, Mima
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Bašić, M. (2019). Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:685671

Bašić, Mima. "Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:685671

Bašić, Mima. "Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:685671

Bašić, M. (2019). 'Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:685671

Bašić M. Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:685671

M. Bašić, "Utjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:685671

Metadata
TitleUtjecaj stresa endoplazmatskog retikuluma na ekspresiju citokina IL-1beta, IL-6, IL-10 i TGF-beta u jetri miša
Title (english)The inffluence of endoplasmic-reticulum stress on cytokine IL-1beta, IL-6, IL-10 and TGF-beta expression in mouse liver
AuthorMima Bašić
MentorMirela Baus Lončar (mentor)
MentorAna Galov (mentor)
Committee memberAna Galov (predsjednik povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2019-09-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Stres endoplazmatskog retikuluma (ER) stanje je narušene homeostaze, okarakteriziran nakupljanjem nesmotanih ili pogrešno smotanih proteina. Tada se pokreće adaptivni mehanizam stanice nazvan odgovor nesmotanih proteina koji sekod sisavaca sastoji od tri glavna signalna puta. Njihova aktivacija rezultira promjenama na transkripcijskoj i translacijskoj razini u svrhu uspostavljanja normalne funkcije ER-a. Kada je stres perzistentan i nepopravljiv, odgovor nesmotanih proteina pokreće apoptozu stanice.Odgovor nesmotanih proteina može aktivirati ključni transkripcijski faktor u modulaciji upalnih odgovora, NF-κB, povećati ekspresiju proupalnih citokina i time upalne procese. Danas se intenzivno istražuje uloga stresa ER-a u patogenezi raznih bolesti. Kako bi se ustanovilo na koji način stres ER-a mijenja ekspresiju gena IL1B, IL6, IL10 i TGFB u tkivu jetre miševa, upoređena je njihova relativna ekspresija u uvjetima stresa ER-a, kod miševa tretiranih tunikamicinom, s relativnom ekspresijom u normalnim fiziološkim uvjetima u kontrolnoj grupi miševa. U tu svrhu izolirana je RNA iz tkiva jetre miševa, reverznom transkripcijom prevedena u cDNA te je analizirana metodom lančane reakcije polimerazom u stvarnom vremenu (qPCR). Analizom rezultata metode qPCR računalnim programom REST ustanovljeno je da stres ER-a uzrokuje statistički značajno povećanje ekspresije IL1B i IL6 te smanjenje ekspresije protuupalnog IL10 u jetri miša.
Abstract (english)
Endoplasmic-reticulum stress is a state of disrupted ER homeostasis, characterized by accumulation of unfolded or misfolded proteins. ER activates adaptive mechanism of cells called unfolded protein response which is comprised of three major signal pathways in mammals. Their activation results in changes on transcriptional and translational level to restore normal ER functions. In case of persistent and irremediable ER stress, unfolded protein response initiates cell apoptosis. Unfolded protein response can activate crucial transcription factor of inflammation, NF-κB, and increase expression of proinflammatory cytokines and processes. Roles of ER stress in pathology of different diseases are being intensively investigated today. To determine how ER stress changes expression of IL1B, IL6, IL10 and TGFB genes in mouse liver, their expression under conditions of ER stress in mice treated with tunicamycin was compared to their expression in normal physiological conditions in control group of mice. For that purpose RNA from mouse liver tissue was isolated, transcribed by reverse transcription into cDNA and analyzed by quantitative polymerase chain reaction (qPCR). Results obtained by qPCR were analysed by REST software. The results showed that ER stress causes statistically significant increase of IL1B and IL6 expression and statistically significant decrease of IL10 expression.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:685671
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-29 10:08:30