prikaz prve stranice dokumenta Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)
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master's thesis
Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:657275
Habazin, Siniša
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Habazin, S. (2014). Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:657275

Habazin, Siniša. "Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:657275

Habazin, Siniša. "Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:657275

Habazin, S. (2014). 'Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:657275

Habazin S. Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:657275

S. Habazin, "Svojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:657275

Metadata
TitleSvojstva i uloga mukoznog sloja na kutikuli oklopljenih dugoživaca (Tardigrada: Heterotardigrada)
Title (english)Properties and function of mucous coat on the cuticle of armoured tardigrades (Tardigrada: Heterotardigrada)
AuthorSiniša Habazin
MentorVesna Svetličić (mentor)
MentorRenata Matoničkin Kepčija (mentor)
Committee memberRenata Matoničkin Kepčija (predsjednik povjerenstva)
Committee memberVlasta Ćosović (član povjerenstva)
Committee memberNenad Buzjak (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberMladen Juračić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Dugoživci su mikroskopski beskralježnjaci koji nepovoljne uvjete u okolišu, poput isušivanja, preživljavaju ulaskom u reverzibilno ametaboličko stanje kriptobioze. Tijelo im prekriva višeslojna kutikula na čijoj je površini mukozni sloj. Cilj ovog rada bio je odrediti sastav, raspored, morfologiju i nanomehanička svojstva mukoznog sloja na kutikuli oklopljenog dugoživca Echiniscus testudo tehnikama bojenja Alcian-modrilom, koloidnim željezom, Nilskim crvenilom i Coomassie briljant plavim, Ramanovom spektroskopijom, pretražnom elektronskom mikroskopijom i mikroskopijom atomskih sila. Materijal mukoznog sloja, u kojem su dokazani mukopolisaharidi, lipidi i proteini, lokaliziran je pretežno u udubljenjima i zonama pregiba dorzalne kutikule. Mikroskopijom atomskih sila oslikana je površina suhe i hidratizirane kutikule u nativnom stanju te je nađeno da se mukozni sloj, građen od kuglastih struktura, prilikom isušivanja skuplja u nepravilne nakupine. Analizom krivulja ovisnosti sile o udaljenosti dobiveni su podaci o nanomehaničkim svojstvima mukoznog sloja i same kutikule. Na temelju rezultata zaključeno je da mukozni sloj ima zaštitnu ulogu i postavljena je hipoteza prema kojoj on djeluje kao brtvilo tanjih dijelova kutikule smanjujući transpiraciju anhidrobionata.
Abstract (english)
Tardigrades are microscopic invertebrates which inhabit terrestrial, freshwater and marine ecosystems. They survive adverse environmental conditions, e.g. dessication, by entering into reversible ametabolic state called cryptobiosis. Body is covered with multilaminated cuticle overlain by mucous coat. Aim of this research was to determine composition, distribution, morphology and nanomechanical properties of mucous coat on the cuticle of armoured tardigrade Echiniscus testudo using Alcian blue, colloidal iron, Nile red and Coomassie brilliant blue staining techniques, as well as Raman spectroscopy, scanning electron microscopy and atomic force microscopy. Mucous coat was found to be composed of mucopolysaccharides, lipids and proteins. It was localized mostly in dorsal epicuticle flexion zones and depressions. Atomic force microscopy imaging revealed that hydrated mucous coat, composed of globular structures, settles into an irregular aggregations upon drying. According to results obtained, protective role of mucous coat was infered; together with hypotesis according to which mucous coat acts as a sealant for thinner regions of the cuticle, thus reducing transpiration of anhydrobionts.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:657275
Study programmeTitle: Enviromental Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-25 08:54:22