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master's thesis
Dinamika proteina Rac1 te njena ovisnost o obliku stanice
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:498904
Ivšić, Blaž
University of Zagreb
Faculty of Science
Department of Physics

Institutional repository: Repository of the Faculty of Science

Cite this document

Ivšić, B. (2019). Dinamika proteina Rac1 te njena ovisnost o obliku stanice (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:498904

Ivšić, Blaž. "Dinamika proteina Rac1 te njena ovisnost o obliku stanice." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:498904

Ivšić, Blaž. "Dinamika proteina Rac1 te njena ovisnost o obliku stanice." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:498904

Ivšić, B. (2019). 'Dinamika proteina Rac1 te njena ovisnost o obliku stanice', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:498904

Ivšić B. Dinamika proteina Rac1 te njena ovisnost o obliku stanice [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:498904

B. Ivšić, "Dinamika proteina Rac1 te njena ovisnost o obliku stanice", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:498904

Metadata
TitleDinamika proteina Rac1 te njena ovisnost o obliku stanice
Title (english)Rac1 dynamics and its dependence on the cell shape
AuthorBlaž Ivšić
MentorIgor Weber (mentor)
MentorNenad Pavin (mentor)
Committee memberNenad Pavin (predsjednik povjerenstva)
Committee memberIgor Weber (član povjerenstva)
Committee memberMirko Planinić (član povjerenstva)
Committee memberNikola Poljak (član povjerenstva)
Committee memberDavor Horvatić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Physics)
Zagreb
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract
Kretanje stanica ovisi o polimerizaciji aktina, a nju reguliraju signalni proteini poput malih GTPaza. Poznato je da je aktivnost tih proteina u eukariotskim stanicama strukturirana u prostorno-vremenske obrasce poput stojnih i putujućih valova koji služe kao predlošci za polarizaciju aktinskog citoskeleta u funkcionalne domene. Dinamika male GTPaze Rac1 prethodno je modelirana pomoću reakcijsko-difuzijskih jednadžbi u jednoj prostornoj dimenziji, te je ustanovljeno da je pojava obrazaca uzrokovana dinamičkim nestabilnostima proizašlim iz nelinearnih interakcija medu proteinima, ali je pritom zanemaren utjecaj promjenjivog oblika stanice na dinamiku sustava. U ovom diplomskom radu poopćen je postojeći model tako da su opisane dvije prostorne dimenzije. Potom je razvijena numerička metoda za ispitivanje dinamike signalnih proteina i istraženo je na koji način oblik stanice utječe na pojavu obrazaca GTPaze Rac1. Teorijski su predvideni različiti prostorno-vremenski obrasci, uključujući putujuće i stojne valove. Također, laserskom pretraženom konfokalnom mikroskopijom eksperimentalno je proučavana dinamika fluorescentno obilježene sonde koja detektira aktivni oblik GTPaze Rac1 u stanicama Dictyostelium tijekom slobodnog kretanja. Dobiveni eksperimentalni rezultati uspoređeni su s teorijskim rezultatima te je zaključeno da uvedeni model dobro opisuje različite obrasce.
Abstract (english)
Cell movement depends on actin polymerization, which is regulated by signal proteins such as small GTPases. It is known that activity of these proteins in eukaryotic cells is structured in spatiotemporal patterns like travelling and stationary waves, which serve as templates for polarization of the actin cytoskeleton into functional domains. A model of Rac1 GTPase dynamics has been previously developed using reaction-diffusion equations in one dimension, which indicated that patterns are formed due to dynamic instabilities originating from non-linear protein interactions. However, a possible influence of cell shape on the system dynamics was not taken into account using this one-dimensional model. In this Diploma thesis we examine the influence of cell geometry on the pattern formation. Laser confocal microscopy was used to obtain data on the dynamics of a fluorecently labeled probe that specifically binds to the active form of Rac1 GTPase in vegetative Dictyostelium cells. Based on the existing model, a numerical method has been developed to investigate signal protein dynamics in two dimensions and determine how the cell shape influences formation of Rac1 GTPase patterns.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:498904
Study programmeTitle: Physics; specializations in: Research
Course: Research
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-01-07 09:23:58