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master's thesis
Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum)
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:022149
Biba, Renata
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Biba, R. (2016). Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:022149

Biba, Renata. "Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum)." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:022149

Biba, Renata. "Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum)." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:022149

Biba, R. (2016). 'Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum)', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:022149

Biba R. Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 July 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:022149

R. Biba, "Utjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:022149

Metadata
TitleUtjecaj nanočestica srebra na pojavu oksidacijskog stresa i ekspresiju proteina u klijancima duhana (Nicotiana tabacum)
Title (english)The effect of silver nanoparticles on oxidative stress appearance and protein expression in tobacco seedlings (Nicotiana tabacum)
AuthorRenata Biba
MentorBiljana Balen (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Nanočestice srebra (AgNP) odlično su protubakterijsko i protugljivično sredstvo i moguće ih je naći u brojnim potrošačkim proizvodima. Zbog potencijalne toksičnosti trenutno su u središtu mnogih istraživanja s ciljem utvrđivanja opasnosti za ekološke sustave i zdravlje ljudi. U ovom je radu istražen utjecaj AgNP na klijance duhana (Nicotiana tabacum), koji je uspoređen s utjecajem ionskog srebra (AgNO3) istih koncentracija. Toksičnost AgNP zabilježena je pri visokim koncentracijama, dok je AgNO3 imao štetno djelovanje i pri nižim koncentracijama. Izmjereni porast razine reaktivnih oblika kisika ukazao je na pojavu oksidacijskog stresa, a promjene u razini malondialdehida i proteinskih karbonila pokazale su oštećenja biološki važnih molekula. Oba oblika srebra uzrokovala su oštećenje molekule DNA, što je bilo izraženije u tretmanima s AgNO3. Antioksidacijski enzimi pokazali su promjenu u aktivnosti i ekspresiji; aktivnost SOD i APX se povećala, a PPX i CAT smanjila u svim tretmanima s AgNP i AgNO3. Došlo je i do promjene u proteinskoj slici, s naglaskom na pojačanoj ekspresiji proteina stresa Hsp70. Rezultati su pokazali da AgNO3 ima jači toksični utjecaj na biljke od AgNP, ali da i AgNP pri povećanim koncentracijama uzrokuju oksidacijski stres i utječu na ekspresiju proteina, zbog čega predstavljaju moguću opasnost za okoliš.
Abstract (english)
Silver nanoparticles (AgNP) are excellent antibacterial and antifungal agents found in various consumer products. Due to their potential toxicity numerous studies are being conducted to determine whether they pose a threat to ecosystems and human health. In this research, the effects of AgNP on tobacco seedlings (Nicotiana tabacum) were analysed and compared with the effects of ionic silver (AgNO3) of the same concentrations. AgNP was toxic when applied in higher concentrations, while AgNO3 showed harmful effects even in lower concentrations. The measured increase of reactive oxygen species indicated appearance of oxidative stress, which was proven by the increase in contents of malondialdehyde and protein carbonyls. Both silver forms caused damage to DNA molecules, which was more pronounced in AgNO3 treatments. Antioxidative enzymes showed a change in activity and expression; the activity of SOD and APX increased, while PPX and CAT activity decreased in all treatments with AgNP and AgNO3. Change in protein patterns was also observed, with emphasis on the expression of stress protein Hsp70. Results indicate that AgNO3 is more toxic for plants than AgNP; however, higher AgNP concentrations also induce oxidative stress and affect protein expression, making them potential environmental hazards.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:022149
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-25 10:10:55