prikaz prve stranice dokumenta Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus
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master's thesis
Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:064928
Biluš, Mirna
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Biluš, M. (2013). Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:064928

Biluš, Mirna. "Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:064928

Biluš, Mirna. "Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:064928

Biluš, M. (2013). 'Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:064928

Biluš M. Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:064928

M. Biluš, "Eukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:064928

Metadata
TitleEukariotske karakteristike izoleucil-tRNA-sintetaze iz bakterije Streptomyces griseus
Title (english)Eukaryotic features of isoleucyl-tRNA synthetase from bacteria Streptomyces griseus
AuthorMirna Biluš
MentorIta Gruić Sovulj (mentor)
Committee memberIta Gruić Sovulj (predsjednik povjerenstva)
Committee memberIvana Ivančić Baće (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Izoleucil-tRNA-sintetaze (IleRS) su enzimi koji kataliziraju nastajanje Ile-tRNAIle. Eukarioti posjeduju IleRS koja se po svojoj primarnoj strukturi, osjetljivosti na antibiotik mupirocin i supstratnoj specifičnosti razlikuje od prokariotskih homologa. Mupirocin snažno inhibira IleRS brojnih bakterija, ali ne i eukariota. Međutim, postoje prokariotske vrste, među njima Streptomyces griseus, čiji se aminokiselinski sljedovi IleRS sravnjuju s eukariotskim. Gen za IleRS iz S. griseus ukloniran je u ekspresijski vektor i eksprimiran u bakteriji Escherichia coli. Funkcionalan protein pročišćen je afinitetnom kromatografijom. Kinetička karakterizacija pokazala je da IleRS iz S. griseus heterologno aminoacilira kvaščevu i E. coli tRNA. Budući da prokariotski tip IleRS (E. coli IleRS) ne aminoacilira kvaščevu tRNAIle, rezultati upućuju da IleRS iz S. griseus nalikuje kvaščevom homologu koji aminoacilira E. coli tRNA. Difuzijski test je pokazao neosjetljivost IleRS iz S. griseus na mupirocin i time ga grupirao s eukariotskim IleRS. Također, pokazano je da IleRS iz S. griseus omogućuje rast E. coli u prisutnosti mupirocina.
Abstract (english)
Isoleucyl-tRNA synthetases (IleRS) catalyze the formation of Ile-tRNAIle. Eukaryotic IleRSs differ from prokaryotic homologues by primary structures, resistance to antibiotic mupirocin and substrate specificity. Mupirocin strongly inhibits IleRS from numerous bacteria, however it does not affect eukaryotes. However, IleRS from some prokaryotes, including Streptomyces griseus, align with the eukaryotic enzymes. The gene for IleRS from S. griseus was cloned and expressed in Escherichia coli. The protein was purified by affinity chromatography. Kinetic characterization established the ability of S. griseus IleRS to heterologously aminoacylate yeast and E. coli tRNAs. As prokaryotic IleRS (E. coli IleRS) does not aminoacylate yeast tRNAIle, the results indicate that S. griseus IleRS resembles the yeast homologue which aminoacylates E. coli tRNA. Diffusion tests showed that like the eukaryotic homologue from yeast, S. griseus IleRS is resistant to mupirocin. Additionally, S. griseus IleRS was able to complement the growth of E. coli in the presence of mupirocin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:064928
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-25 11:46:03