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master's thesis
Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:021510
Čorić, Marta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Čorić, M. (2013). Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:021510

Čorić, Marta. "Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:021510

Čorić, Marta. "Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:021510

Čorić, M. (2013). 'Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:021510

Čorić M. Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:021510

M. Čorić, "Biokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:021510

Metadata
TitleBiokemijska karakterizacija produkta gena At4g01150 iz biljke Arabidopsis thaliana
Title (english)Biochemical characterization of At4g01150 gene product of Arabidopsis thaliana
AuthorMarta Čorić
MentorHrvoje Fulgosi (mentor)
MentorŽeljka Vidaković-Cifrek (mentor)
Committee memberŽeljka Vidaković-Cifrek (predsjednik povjerenstva)
Committee memberHrvoje Fulgosi (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-02-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fotosinteza je značajan biološki proces u kojem se djelovanjem Sunčevog zračenja anorganske tvari (voda i ugljikov dioksid) prevode u složene organske spojeve i kisik, koji su bitni za prehranu skoro svih živih bića na Zemlji. Proteini uključeni u glavne reakcije fotosinteze najvećim su dijelom već otkriveni i njihove su funkcije istražene, no nedavnim sekvenciranjem genoma biljke Arabidopsis thaliana i brojnim sustavnim istraživanjima genoma biljaka te ekspresije proteina, pronađeni su novi proteini eksprimirani u biljnim kloroplastima, čije funkcije još nisu razjašnjene. Jedan od tih proteina je i produkt gena At4g01150 iz biljke A. thaliana, nazvan CAASE. Proučavanjem pojedinih odjeljaka kloroplasta pronađen je na vanjskoj i unutarnjoj membrani kloroplasta, u tilakoidima, te u plastoglobulima, no njegova funkcija do danas je nepoznata. Ovo istraživanje posvećeno je biokemijskim analizama proteina CAASE te dobivanju biljaka bez ekspresije CAASE-a. Protutijelo koje prepoznaje protein CAASE omogućilo je određivanje točne lokalizacije CAASE-a u biljnim tkivima koji proizvode fotosintezu, tj. sadrže kloroplaste, te u stroma tilakoidima kloroplasta. Biljke homozigoti za mutirani gen At4g01150 ipak proizvode male količine proteina CAASE, te se ne mogu koristiti u daljnjim istraživanjima. Zbog potreba izolacije i daljnjih istraživanja proteina CAASE konstruirana je rekombinantna molekula CAASE, s afinitetnim biljegom HA na C-terminalnom kraju proteina.
Abstract (english)
Oxygen photosynthesis is an important biological process in which the effect of solar energy can translate inorganic substances (water and carbon dioxide) into complex organic compounds, thus providing food and oxygen for all living organisms. Proteins involved in the main reactions of photosynthesis are mostly known, as well as their functions, but the recent Arabidopsis thaliana genome sequencing, and a number of systematic plant genome and protein expression studies, discovered new proteins localized in plant chloroplasts, whose functions are unclear. One of these proteins is the product of At4g01150 gene, named CAASE. The study of different compartments of chloroplasts showed that CAASE is present, on the outer and inner membranes of chloroplasts, thylakoids and in plastoglobules, but its function is so far unknown. In this study, the usage of the antibody that recognizes endogenous CAASE enabled the precise localization of the protein in photosynthetic plant tissues and, moreover, in the stroma thylakoids. However, the plants established to be devoid of CAASE still produced this protein, making it difficult to observe mutant phenotype. In order to investigate CAASE further, a vector for affinity purification was constructed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:021510
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-25 12:51:30