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master's thesis
Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:205855
Pregiban, Kristina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Pregiban, K. (2020). Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:205855

Pregiban, Kristina. "Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:205855

Pregiban, Kristina. "Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:205855

Pregiban, K. (2020). 'Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:205855

Pregiban K. Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:205855

K. Pregiban, "Ispitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:205855

Metadata
TitleIspitivanje interakcije novih derivata benzoksantena s dvočlanim DNA i RNA, te albuminom iz humanog seruma spektroskopskim i kalorimetrijskim metodama
Title (english)Study of interactions of new benzoxanthene derivatives with double-stranded DNA and RNA, and albumin from human serum by spectroscopic and calorimetric methods
AuthorKristina Pregiban
MentorLidija-Marija Tumir (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberSanda Rončević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-02-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U okviru ovog diplomskog rada metodama UV/Vis i fluorescencijske spektroskopije, spektroskopski su okarakterizirane vodene otopine novih derivata benzoksantena (CT-A19, CT-A22, CT-A23 i CT-A24). UV/Vis i fluorescencijski spektri pokazali su linearnost u određenom koncentracijskom području u skladu s Beer-Lambertovim zakonom. Koristeći fluorimetrijske titracije, spojevi su pokazali veći afinitet prema albuminu iz humanog seruma (HSA), nego prema polinukleotidima. Konstante stabilnosti kompleksa ispitivanih benzoksantenskih spojeva s polinukleotidima i proteinom izračunate su pomoću Scatchardove jednadžbe metodom nelinearne regresije. Mjerenjem temperature mekšanja i spektroskopijom cirkularnog dikroizma pokazano je da vezanje spojeva ne utječe značajno na stabilnost i promjenu sekundarne strukture polinukleotida. Diferencijalnom pretražnom kalorimetrijom istraženo je vezanje benzoksantena na protein HSA, pri čemu je pokazano da spoj CT-A22 najviše utječe na termičku stabilinost sekundarne strukture proteina.
Abstract (english)
The thesis describes characterisation of water solutions of new benzoxanthen derivatives (CT- A19, CT-A22, CT-A23 and CT-A24) by UV/Vis and fluorescence spectroscopic methods. UV/Vis and fluorescence spectra of compounds showed linear dependence on compound’s concentrations according to the Beer-Lambert law. Examined compounds showed higher affinity of for human serum albumin (HSA) than for the polynucleotides obtained by fluorimetric titrations. The stability constants of the complexes of the studied benzoxanthenic compounds with polynucleotides and proteins were calculated using the Scatchard equation. Thermal melting measurements and circular dichroism spectroscopy showed no significant effect on double helix stability and they didn’t change the polynucleotide secondary structure. Differential scanning calorimetry (DSC) was used to examine the influence of benzoxanthene compound to HSA thermal stability. Results showed highest CT-A22 impact on the protein thermal stability.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:205855
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-02-17
Terms of useLicence In copyright icon
Created on2020-02-26 09:10:50