prikaz prve stranice dokumenta Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi
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master's thesis
Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:780683
Bogut, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Bogut, A. (2014). Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:780683

Bogut, Ana. "Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:780683

Bogut, Ana. "Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:780683

Bogut, A. (2014). 'Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:780683

Bogut A. Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:780683

A. Bogut, "Učinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:780683

Metadata
TitleUčinak proteina FGF i HMGB1 na preživljenje i proliferaciju živčanih matičnih stanica u kulturi
Title (english)The effect of FGF and HMGB proteins on the survival and proliferation potential of neural stem cells in culture
AuthorAna Bogut
MentorDinko Mitrečić (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-11-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Ovaj rad opisuje utjecaj FGF i HMGB1 molekula na živčane matične stanice u kulturi (NSC). Primjenom metode magnetofekcije živčane matične stanice transfecirane su plazmidima s FGFR1 receptorom. Osim NSC stanica u eksperimentima su korištene i embrionalne matične stanice bubrega (HEK293T). Pokazalo se da FGF utječe pozitivno na preživljenje HEK293T stanica s transfeciranim plazmidima u usporedbi sa HEK stanicama koje nisu transfecirane. HMGB1 je smanjio pozitivan učinak FGF-a u kulturi HEK293T stanica. NSC stanice u svojem mediju već imaju FGF te je postignuto bolje preživljenje NSC stanica transfeciranih FGFR1-EGFP plazmidom. Korištenjem dvostruko veće količine plazmida u odnosu na magnetne čestice postigla se dvostruko bolja uspješnost transfekcije metodom magnetofekcije u usporedbi sa prethodnim istraživanjima. HMGB1 je na njihovo preživljenje djelovao negativno kao i kod HEK293T stanica.
Abstract (english)
The aim of this study was to describe the effects of FGF and HMGB1 molecules on cultured neural stem cells (NSCs). Using magnetofection, NSCs were transfected with plasmides containing the FGFR1 receptor. Along with NSCs, embryonal kidney stem cells (HEK293T) were also used. The results show that FGF positively affects the survival of HEK293T cells, which were transfected with plasmides, as opposed to non-transfected cells. The HMGB1 molecule reduced the positive effect of FGF in cultured HEK293T cells. NSCs were already grown in a medium containing FGF. The ones transfected with the FGFR1-EGFP plasmide showed increased survival. Using twice as many plasmides as magnet particles resulted in a twofold increase in magnetofection success rate, compared to previous studies. At the same time, HMGB1 affected their survival negatively, just as in HEK293T cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:780683
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 07:03:11