prikaz prve stranice dokumenta Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9
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master's thesis
Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:722408
Božić, Tanja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Božić, T. (2014). Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:722408

Božić, Tanja. "Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:722408

Božić, Tanja. "Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:722408

Božić, T. (2014). 'Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:722408

Božić T. Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:722408

T. Božić, "Utjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:722408

Metadata
TitleUtjecaj ljudskih proteina alfa-enolaze i MBP-1 na stabilizaciju telomera u mišjim fibroblastima A9
Title (english)Influence of human proteins alfa-enolase and MBP-1 on telomere stabilization in mice fibroblasts A9
AuthorTanja Božić
MentorIvica Rubelj (mentor)
MentorMaja Matulić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberIvica Rubelj (član povjerenstva)
Committee memberMirjana Kalafatić (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-02-17, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Mišji fibroblasti A9 imaju telomere duljine ~9 kb. Kada se u stanice A9 unese ljudski kromosom 1, ili ljudski proteini kodirani s tog kromosoma, α-enolaza ili MBP-1, duljina telomera se skrati na ~4 kb. Promjena ravnotežne točke na kraće duljine može upućivati na promjenu telomerne konformacije te veću stabilnost njihove strukture. Zato sam provjerila duljine telomera u normalnim i hibridnim fibroblastima A9, te utvrdila nastaju li u tim stanicama t-krugovi koji su inače indikator nestabilnosti telomera. Duljine telomera sam provjerila analizom po Southernu, a t-krugove sam istražila 2D gel elektroforezom DNA i tehnikom umnažanja signala t-krugova kotrljajućom sintezom. Rezultati su pokazali da su telomere u hibridnim stanicama A9 skraćene te da u njima dolazi do značajnog smanjenja nastanka t-krugova. Činjenica da unošenje ljudskog kromosoma 1 te ljudskih proteina α-enolaze i MBP-1 u stanice A9 uzrokuje skraćenje telomera, znači da su telomere u ovim stanicama i stabilnije, zbog čega po svojoj dinamici nalikuju na ljudske telomere.
Abstract (english)
Mice fibroblasts A9 have telomeres ~9 kb in length. Introducing the human chromosome 1 or human proteins coded from the same chromosome, α-enolase or MBP-1 into A9 cells, causes telomere lenght reduction to ~4 kb. Change in the equilibrium point to shorter lengths could suggest a change in telomere conformation and their increased stability. For that reason I have verified the telomere length in normal and hybrid fibroblasts A9 and determined the occurrence of t-circles which indicate telomere instability. Telomere length was verified by Southern blot analysis, and t-circles were analysed by 2D gel electrophoresis and by t-circle amplification assay based on rolling circle amplification. Results demonstrate telomere shortening in hybrid A9 cells and significantly lower occurance of t-circles. Introduction of human chromosome 1 or human proteins α-enolase or MBP-1 into A9 cells causes telomere shortening and their stabilization and therefore their telomere dynamics resembles the human.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:722408
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 08:42:22