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master's thesis
Međudjelovanje kolesterola i antioksidativnog sustava
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:827048
Dominko, Kristina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Dominko, K. (2014). Međudjelovanje kolesterola i antioksidativnog sustava (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:827048

Dominko, Kristina. "Međudjelovanje kolesterola i antioksidativnog sustava." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:827048

Dominko, Kristina. "Međudjelovanje kolesterola i antioksidativnog sustava." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:827048

Dominko, K. (2014). 'Međudjelovanje kolesterola i antioksidativnog sustava', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:827048

Dominko K. Međudjelovanje kolesterola i antioksidativnog sustava [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:827048

K. Dominko, "Međudjelovanje kolesterola i antioksidativnog sustava", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:827048

Metadata
TitleMeđudjelovanje kolesterola i antioksidativnog sustava
Title (english)Interactio of cholesterol and antioxidant system
AuthorKristina Dominko
MentorSilva Katušić Hećimović (mentor)
MentorDomagoj Đikić (mentor)
Committee memberDomagoj Đikić (predsjednik povjerenstva)
Committee memberSilva Katušić Hećimović (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberVesna Benković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-02-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj rada bio je odrediti kako prehrana bogata kolesterolom utječe na koncentraciju kolesterola i razinu oksidativnog stresa u jetri i mozgu miševa (C57BL). Također, cilj rada je bio na staničnom modelu CHOwt i NPC1null (stanice CHO s deletiranim genom za protein NPC1 koje nakupljaju kolesterol) odrediti antioksidativnu obranu stanica pri normalnim uvjetima i u uvjetima povećanog oksidativnog stresa. U pokusu je mjerena lipidna peroksidacija, koncentracija ukupnog glutationa te aktivnost antioksidativnih enzima superoksid dismutaze i katalaze. Rezultati su pokazali da je mozak zaštićeniji od jetre od oksidativnog stresa nastalog kao posljedica hiperkolesterolemije. Stanice NPC1null imaju povećani oksidativni stres u normalnim uvjetima. Pri povećanom oksidativnom stresu stanice NPC1null imaju oslabljenu antioksidativnu obranu. Na životinjskom i staničnom modelu je pokazano da su na oksidativni stres uzrokovan nakupljanjem kolesterola najosjetljiviji mitohondriji, a najpogođeniji antioksidans je superoksid dismutaza.
Abstract (english)
Aim of this study was to determine how cholesterol-rich diet influences the cholesterol concentration and oxidative stress levels in mice (C57BL) liver and brain. Also, aim was to determine the levels of antioxidant defense systems in cell model CHOwt and NPC1null (CHO cells with deleted gene for NPC1 protein which accumulate cholesterol) in normal conditions and under higher levels of oxidative stress. In this study the amount of lipid peroxidation, concentration of total glutathione and activity of antioxidant enzymes superoxide dismutase and catalase was measured. Results have shown that the brain is more protected from oxidative stress caused by hypercholesterolemia than the liver. Cells NPC1null have higher oxidative stress levels in normal conditions. Under conditions of higher oxidative stress, cells NPC1null have lower antioxidant defense. In animal and cell models, mitochondria are especially susceptible to oxidative stress caused by cholesterol accumulation and the most affected antioxidant is superoxide dismutase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:827048
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 08:57:42