prikaz prve stranice dokumenta Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro
Access restricted to students and staff of home institution
master's thesis
Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:799397
Jadrijević, Ivana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jadrijević, I. (2014). Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:799397

Jadrijević, Ivana. "Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:799397

Jadrijević, Ivana. "Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:799397

Jadrijević, I. (2014). 'Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:799397

Jadrijević I. Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:799397

I. Jadrijević, "Razmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:799397

Metadata
TitleRazmnožavanje krute broćike (Galium rubioides L.) u uvjetima in vitro
Title (english)Micropropagation of European bedstraw (Galium rubioides L.)
AuthorIvana Jadrijević
MentorBranka Pevalek-Kozlina (mentor)
Committee memberBranka Pevalek-Kozlina (predsjednik povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberZdravko Dolenec (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-02-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Istraživano je razmnožavanje krute broćike (Galium rubioides L.), kritično ugrožene vrste u uvjetima in vitro. Početni eksplantanti bili su izdanci klijanaca uzgojenih iz sjemenki u uvjetima in vitro. Stopa umnožavanja bila je veća na podlogama s punom koncentracijom makroelemenata. Povećanje koncentracije BA u podlozi MS pozitivno je utjecalo na stope umnožavanja u svim supkulturama. Najveći broj izdanaka razvijao se na podlozi MS s dodatkom 1,0 μM BA. Na podlozi MS½ najveći broj izdanaka razvijao se kada nije bio dodan BA. Visine prvih izdanaka povećavale su se s povećanjem koncentracije BA. Visine drugih izdanaka bila su znatno manje. Na podlogama za umnožavanje neki izdanci su se zakorjenjivali, a na bazi nekih razvilo se kalusno tkivo. Izdanci su uspješno zakorjenjivani na podlozi MS uz dodatak različitih vrsta i koncentracija auksina. Zakorjenjivanje je bilo najbolje na podlogama s 2,5 ili 4,9 μM IBA. Postupak aklimatizacije na vanjske uvjete bio je vrlo uspješan (90%).
Abstract (english)
The micropropagation possibility for European bedstraw (Galium rubioides L.), a critically endangered species was investigated. Shoots originated from aseptically germinated seeds were used for culture initiation. The multiplication rate per explant was higher on full MS medium. Increasing the concentration of BA in medium had a positive effect on multiplication rates in all subcultures. On MS full medium the highest shoot number was observed with addition of 1,0 μM BA while on half-strength MS medium the highest shoot number was observed when no BA was added. The length of first shoots increased with increasing concentration of BA. The second shoots were significantly shorter. Some shoots incubated on multiplication media rooted spontaneously. Also, in some cultures development of callus tissue was observed. Shoots were successfully rooted on MS medium containing three different kinds of auxins in various concentrations. The best rooting was observed on media supplemented with 2,5 or 4,9 μM IBA. Acclimatization of shoots to outdoor conditions was very succesfull with a surviving rate of 90%.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:799397
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 09:23:03