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Scientific paper - Original scientific paper
The mitotic spindle is chiral due to torques within microtubule bundles
Nature Communications, 9 (2018); 3571. https://doi.org/10.1038/s41467-018-06005-7
Novak, Maja; Polak, Bruno; Simunić, Juraj; Boban, Zvonimir; Kuzmić, Barbara; Thomae, Andreas W; Tolić, Iva Marija; Pavin, Nenad

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Novak, M., Polak, B., Simunić, J., Boban, Z., Kuzmić, B., Thomae, A. W. ... Pavin, N. (2018). The mitotic spindle is chiral due to torques within microtubule bundles. Nature Communications, 9.. doi: 10.1038/s41467-018-06005-7

Novak, Maja, et al. "The mitotic spindle is chiral due to torques within microtubule bundles." Nature Communications, vol. 9, 2018. https://doi.org/10.1038/s41467-018-06005-7

Novak, Maja, Bruno Polak, Juraj Simunić, Zvonimir Boban, Barbara Kuzmić, Andreas W Thomae, Iva Marija Tolić and Nenad Pavin. "The mitotic spindle is chiral due to torques within microtubule bundles." Nature Communications 9 (2018). https://doi.org/10.1038/s41467-018-06005-7

Novak, M., et al. (2018) 'The mitotic spindle is chiral due to torques within microtubule bundles', Nature Communications, 9. doi: 10.1038/s41467-018-06005-7

Novak M, Polak B, Simunić J, Boban Z, Kuzmić B, Thomae AW, and sur.. The mitotic spindle is chiral due to torques within microtubule bundles. Nature Communications [Internet]. 2018 [cited 2024 May 27];9. doi: 10.1038/s41467-018-06005-7

M. Novak, et al., "The mitotic spindle is chiral due to torques within microtubule bundles", Nature Communications, vol. 9, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:446605. [Accessed: 27 May 2024]

Metadata
Title (english)The mitotic spindle is chiral due to torques within microtubule bundles
AuthorMaja Novak
MBZ: 344456
AuthorBruno Polak
AuthorJuraj Simunić
MBZ: 322860
AuthorZvonimir Boban
AuthorBarbara Kuzmić
MBZ: 362291
AuthorAndreas W Thomae
AuthorIva Marija Tolić
MBZ: 260543
AuthorNenad Pavin
MBZ: 204800
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract (english)
Mitosis relies on forces generated in the spindle, a micro- machine composed of microtubules and associated proteins. Forces are required for the congression of chromosomes to the metaphase plate and their separation in anaphase. However, besides forces, torques may exist in the spindle, yet they have not been investigated. Here we show that the spindle is chiral. Chirality is evident from the finding that microtubule bundles in human spindles follow a left-handed helical path, which cannot be explained by forces but rather by torques. Kinesin-5 (Kif11/Eg5) inactivation abolishes spindle chirality. Our theoretical model predicts that bending and twisting moments may generate curved shapes of bundles. We found that bundles turn by about −2 deg µm−1 around the spindle axis, which we explain by a twisting moment of roughly −10 pNµm. We conclude that torques, in addition to forces, exist in the spindle and determine its chiral architecture.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleNature Communications
Numberingvol. 9, 3571
p-ISSN2041-1723
DOIhttps://doi.org/10.1038/s41467-018-06005-7
URN:NBNurn:nbn:hr:217:446605
Publication2018
Document URLhttps://www.nature.com/articles/s41467-018-06005-7
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. Published: 03 September 2018.
Created on2020-03-01 12:27:14