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master's thesis
Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni'
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:439407
Pavelić, Katarina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Pavelić, K. (2020). Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni' (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:439407

Pavelić, Katarina. "Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni'." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:439407

Pavelić, Katarina. "Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni'." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:439407

Pavelić, K. (2020). 'Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni'', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:439407

Pavelić K. Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni' [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:439407

K. Pavelić, "Učinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni'", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:439407

Metadata
TitleUčinak indol-3-maslačne kiseline na bakteriju 'Candidatus Phytoplasma pruni'
Title (english)The effect of indole-3-butyric acid on 'Candidatus Phytoplasma pruni'
AuthorKatarina Pavelić
MentorMirna Ćurković-Perica (mentor)
Committee memberInes Radanović (predsjednik povjerenstva)
Committee memberDubravka Matković-Čalogović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Bakterije roda 'Candidatus Phytoplasma' su unutarstanični, biljni patogeni bez stanične stijenke iz razreda Mollicutes. Uzrokuju bolesti brojnih biljaka širom svijeta. Svrha ovog rada bila je istražiti učinak egzogenog biljnog regulatora rasta indol-3-maslačne kiseline (IBA) na bakteriju 'Candidatus Phytoplasma pruni' (soj KVI) i domaćinsku biljku madagaskarski zimzelen (Catharanthus roseus (L.) G. Don). Zdravi i bakterijom zaraženi izdanci madagaskarskog zimzelena dugoročno su održavani u kulturi tkiva in vitro na hranidbenoj podlozi s dodanim biljnim regulatorom rasta indol-3-maslačnom kiselinom (IBA) ili benzilamunopurinom (BAP). Tijekom tri supkulture (13., 14. i 15.) mjerene su i uspoređivane visine izdanaka. Rezultati su pokazali da je biljni regulator rasta IBA povoljno utjecao na produžni rast fitoplazmom zaraženih izdanaka madagaskarskog zimzelena. Prisutnost bakterije 'Candidatus Phytoplasma pruni' (soj KVI) u zaraženim izdancima madagaskarskog zimzelena provjerena je „ugniježđenom“ lančanom reakcijom polimerazom. Fitoplazma nije detektirana u 83% izdanaka tretiranih IBA-om, što pokazuje da IBA ima učinak na tu bakteriju.
Abstract (english)
Bacteria of the genus 'Candidatus Phytoplasma' are intercellular, wall-less plant pathogens from the class Mollicutes. They cause diseases in numerous plants worldwide. The purpose of this study was to investigate the effect of the exogenous plant growth regulator indole-3-butyric acid (IBA) on the 'Candidatus Phytoplasma pruni' (strain KVI) and the host plant Madagascar periwinkle (Catharanthus roseus (L.) G. Don). Healthy and infected shoots of Madagadscar periwinkle were grown in vitro on the nutrient medium containing growth regulator indol-3-butyric acid (IBA) or benzylaminopurine (BAP). During three subcultures (13., 14. and 15.) heights of their shoots were monitored and compared. The results have shown that plant growth regulator IBA had positive effect on the elongation of phytoplasma-infected Madagascar periwinkle shoots. The presence of 'Candidatus Phytoplasma pruni' (strain KVI) in the infected shoots of the Madagascar Periwinkle was tested by nested Polymerase Chain Reaction. Phytoplasma was not detected in 83% of the shoots treated with IBA, which shows that IBA has the effect on that bacterium.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:439407
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-03-16 10:27:11