prikaz prve stranice dokumenta Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima
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master's thesis
Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:828439
Lazić, Aleksandar
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Lazić, A. (2014). Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:828439

Lazić, Aleksandar. "Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:828439

Lazić, Aleksandar. "Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:828439

Lazić, A. (2014). 'Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:828439

Lazić A. Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 July 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:828439

A. Lazić, "Stabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:828439

Metadata
TitleStabilnost genoma zebrica (Danio rerio Hamilton 1822) nakon izlaganja modelnim genotoksikantima
Title (english)Genome stability of zebrafish (Danio rerio Hamilton 1822) after exposure to model genotoxicants
AuthorAleksandar Lazić
MentorGoran Klobučar (mentor)
Committee memberGoran Klobučar (predsjednik povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberMirjana Pavlica (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-02-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Oštećenja molekule DNA, koja nastaju kao posljedica izlaganja toksičnim tvarima, mogu u konačnici utjecati na zdravlje i preživljavanje organizama. Stoga je bitno razvijati i testirati osjetljive molekularno citogenetičke metode za ranu detekciju i procjenu takvih promjena. Cilj ovog rada bio je procijeniti oštećenja molekule DNA u zebricama (Danio rerio) izloženim modelnim genotoksikantima (benzo[a]piren (B[a]P), etilmetanosulfonat (EMS)) te utvrditi dugotrajnost takvih oštećenja. Primarno oštećenje DNA detektirano je metodom Komet-testa dok su dugotrajne posljedice praćene metodom umnoženih polimorfnih DNA biljega (engl. amplified fragment length polymorphism, AFLP). Rezultatima ovog istraživanja dokazana su primarna oštećenja DNA uzrokovana modelnim genotoksikantima te perzistentnost promjena u genomu zebrica i godinu dana nakon izlaganja genotoksikantima, te je potvrđena pogodnost korištenja zebrica kao modelnih organizama u istraživanjima genotoksičnosti.
Abstract (english)
DNA damage as a consequence of toxicant exposure may in the long run influence health and survival of the organisms. Therefore, it is of paramount importance to develop and test sensitive molecular cytogenetic methods for early detection and assessment of these changes. The aim of this study was to assess DNA damage in zebrafish (Danio rerio) exposed to model genotoxicants (Benzo[a]pyrene (B[a]P), Ethyl methanesulfonate (EMS)) and to determine the longevity of such damage. The primary DNA damage was detected using Comet assay whereas the long-term effects were monitored using the AFLP (amplified fragment length polymorphism) method. The results of this study demonstrated the primary DNA damage caused by model genotoxicants as well as persistence of alterations in the zebrafish genome even one year after the end of the genotoxic exposure. Zebrafish was confirmed as suitable model organism in studies of genotoxicity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:828439
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 10:19:11