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master's thesis
Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:973591
Medved, Magdalena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Medved, M. (2020). Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:973591

Medved, Magdalena. "Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:973591

Medved, Magdalena. "Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:973591

Medved, M. (2020). 'Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:973591

Medved M. Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 May 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:973591

M. Medved, "Utvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:973591

Metadata
TitleUtvrđivanje i analiza haplotipova kontrolne regije mitohondrijske DNA dobrih dupina (Tursiops truncatus, Montagu, 1821) Jadranskog mora
Title (english)Determination and analysis of the mitochondrial DNA control region haplotypes of the bottlenose dolphin (Tursiops truncatus, Montagu, 1821) from the Adriatic Sea
AuthorMagdalena Medved
MentorAna Galov (mentor)
MentorMartina Đuras (mentor)
Committee memberAna Galov (predsjednik povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-02-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Dobri dupin (Tursiops truncatus Montagu, 1821) jedina je vrsta morskog sisavca koja u Jadranu obitava cijelu godinu. Populacija dupina, ne samo Jadrana već i globalno, sve je ugroženija djelovanjem klimatskih promjena, litoralizacije, izlovom ribe, sve intenzivnijim morskim prometom. Istraživanja dupina Sredozemnog mora malobrojna su, a čine osnovu za daljnje postupke u zaštiti i konzervaciji populacija. U ovom radu utvrđeni su i analizirani haplotipovi kontrolne regije mitohondrijske DNA populacije dobrih dupina Jadranskog mora te uspoređeni s ostalim objavljenim haplotipovima. Nakon izolacije DNA provela sam PCR reakciju, purifikaciju te sekvenciranje kontrolne regije, a zatim i računalne analize te izradila mreže. Istraživanje je pokazalo da populacija dobrih dupina Jadranskog mora ima 13 haplotipova kontrolne regije mtDNA od kojih su u ovom radu utvrđena dva nova (DD12 i DD13). Haplotipska raznolikost (Hd) iznosi 0,6831 a nukleotidna (π) 0,01085 što ukazuje na relativno visoku razinu genetičke raznolikosti. Mreža haplotipova dobivenih u ovom radu i onih preuzetih s GenBanka ne pokazuje jasnu filogeografsku strukturiranost populacija dobrih dupina Jadranskog, Sredozemnog, Crnog mora te sjeveroistočnog Atlantika.
Abstract (english)
The bottlenose dolphin (Tursiops truncatus Montagu, 1821) is the only species of marine mammals that lives in the Adriatic Sea throughout the entire year. The population of dolphins, not only in the Adriatic but also globally, is increasingly threatened by climate change, littoralisation, fishing and growing marine traffic. Studies of the Mediterranean Sea dolphins are few, and form the basis for further action for the protection and conservation of their populations. In this graduation thesis, the mitochondrial DNA control region haplotypes of the bottlenose dolphin population in the Adriatic Sea have been identified and compared with other published haplotypes. After isolating the DNA, I performed the PCR reaction, purification and sequencing of the control region, followed by computer analysis, after which I constructed the haplotype networks. The study has shown that the common bottlenose dolphin population of the Adriatic Sea has 13 mtDNA control region haplotypes, of which two new haplotypes (DD12 and DD13) have been identified in this graduation thesis. The haplotype diversity (Hd) is 0,6831 and nucleotide diversity (π) is 0,01085, which points to a relatively high level of genetic diversity. The haplotype network showing a comparison of haplotypes determined in this graduation thesis and haplotypes downloaded from GenBank does not show a clear phylogeographic structure of populations of the Adriatic, the Mediterranean, the Black Sea and the northeast Atlantic.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:973591
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-02-18
Terms of useLicence In copyright icon
Created on2020-06-09 09:48:14