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master's thesis
Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:918563
Anić, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Anić, P. (2020). Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:918563

Anić, Petra. "Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:918563

Anić, Petra. "Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:918563

Anić, P. (2020). 'Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:918563

Anić P. Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:918563

P. Anić, "Metiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:918563

Metadata
TitleMetiliranost promotora gena imunosnog sustava u tumorima glave i vrata čovjeka
Title (english)Methylation of immune gene promoters in human head and neck cancer
AuthorPetra Anić
MentorNina Milutin Gašperov (mentor)
MentorInga Marijanović (sumentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
Committee memberMirna Ćurković Perica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-02-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Udio tumora glave i vrata koji se mogu povezati s infekcijom humanim papiloma virusima (HPV, Human papilloma virus) raste. Jedan od potencijalnih čimbenika odgovornih za nastanak tumora kod infekcije HPV-om je promjena stupnja metiliranosti promotora gena koji igraju ključnu ulogu u imunosnom odgovoru. U radu je istražena razlika u metiliranosti promotora gena: EDARADD, IL12RB, MARCO, HLA DPB, GBP, SIGLEC i HAVCR2 u obriscima zdrave oralne sluznice naspram svježeg tkiva oralnih i orofarinegealnih tumora čovjeka. Ispitano je i prisustvo infekcije HPV-om u svakom pojedinom uzorku. Za određivanje razlike u metiliranosti promotora navedenih gena, prethodno izolirana DNA je podvrgnuta kemijskoj modifikaciji natrijevim bisulfitom te umnožena metodom lančane reakcije polimerazom specifične za metilaciju. Analizirani geni pokazali su hipometiliranost kod uzoraka oralnih i orofaringealnih tumora glave i vrata u usporedbi s normalnim tkivom. Također, udio nemetiliranih promotora navedenih gena bio je veći kod HPV-pozitivnih tumora nego kod HPV-negativnih tumora, što ukazuje da je njihova aktivacija demetiliranjem najvjerojatnije odgovor na onkogene E6 i E7 HPV-a. Razlike u metiliranosti navedenih gena mogu biti pokazatelji prognoze te progresije pojedinih tumora glave i vrata te, po mogućnosti, odgovor na pitanje zašto kod nekih ljudi dolazi do razvoja ovih tumora, dok kod drugih ne, bez obzira na infekciju HPV-om.
Abstract (english)
The proportion of human head and neck (HNC) cancer that can be attributable to human papilloma virus (HPV) infection is growing. A potential factor responsible for the emergence of HPV-positive HNC is a change in the degree of methylation of gene promoters that play a key role in the immune response. In this study, we investigated the difference in methylation of EDARADD, IL12RB, MARCO, HLA DPB, GBP, SIGLEC and HAVCR2 gene promoters, in samples of healthy oral mucosa versus samples of oral and oropharyngeal tumors. The presence of HPV infection in samples was examined. To determine the difference in methylation of those gene promotors, isolated DNA was subjected to chemical modification by sodium bisulfite and amplified by methylation-specific PCR method. Analyzed genes showed hypomethylation in samples of oral and oropharyngeal tumors compared to normal tissue. Proportion of unmethylated promoters is higher in HPV-positive tumors than in HPV-negative tumors, indicating that their activation by demethylation is most likely a response to HPV oncogenes E6 and E7. These differences may be indicators of the prognosis and the progression of individual HNC and answer to the question why some people develop HNC, while others not, regardless of HPV infection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:918563
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-02-21
Terms of useLicence In copyright icon
Created on2020-06-09 09:55:12