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master's thesis
Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:667753
Foglar, Nika
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Foglar, N. (2020). Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:667753

Foglar, Nika. "Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:667753

Foglar, Nika. "Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:667753

Foglar, N. (2020). 'Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:667753

Foglar N. Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 May 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:667753

N. Foglar, "Utjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:667753

Metadata
TitleUtjecaj proteina TRNP1 na proliferaciju primarne kulture stanica korteksa iz E14 miša
Title (english)Effect of Trnp1 orthologs on proliferation of E14 mouse cortical primary culture
AuthorNika Foglar
MentorMiriam Esgleas (mentor)
MentorInga Marijanović (sumentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-02-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Through the evolution of mammals, the brain size has increased by folding the neocortex. The cause for differences in folding between species could be different regulation of some genes. Trnp1 gene overexpression causes self-renewal of neuronal stem cells in vitro, while in vivo knockdown leads to folding of the cortex in mice. Rate of evolutionary mutation was calculated for Trnp1 orthologs, and correlation between these rates and degree of gyrification was noticed. To determine if evolutionary mutations of Trnp1 have different effect on the self-renewal capacity of cortical primary culture cells, six Trnp1 orthologs were overexpressed in these cells. The six species were Mus musculus, Otolemur garnettii, Macaca mulatta, Mustela putorius, Homo sapiens and Tursiops truncates. Analysis showed that all Trnp1 orthologs increase proliferation, but the effect of each ortholog was similar. Therefore, it is unlikely that evolutionary mutations of Trnp1 caused the differences in folding between these species.
Abstract (croatian)
Kroz evoluciju sisavaca, veličina mozga se povećavala stvaranjem nabora u mozgu. Uzrok različite naboranosti mozga između sisavaca mogao bi biti različita regulacija nekih gena. Prekomjerna ekspresija gena Trnp1 uzrokuje samoobnavljanje živčanih matičnih stanica in vitro, dok in vivo smanjenje ekspresije uzrokuje stvaranje nabora u korteksu miša. Brzina evolucijskih mutacija je izračunata za Trnp1 ortologe te je primijećena korelacija između te brzine i stupnja naboranosti mozga. Kako bi se odredilo imaju li evolucijske mutacije gena Trnp1 različiti efekt na sposobnost samoobnavljanja stanica primarne kulture korteksa, šest ortologa Trnp1 je eksprimirano u stanicama. Odabrane vrste su bile Mus musculus, Otolemur garnettii, Macaca mulatta, Mustela putorius, Homo sapiens and Tursiops truncates. Analiza je pokazala da svi ortolozi Trnp1 povećavaju sposobnost proliferacije, ali su ortolozi imali podjednak učinak. Stoga je malo vjerojatno da su evolucijske mutacije gena Trnp1 uzrokovale razlike u stupnju naboranosti mozga između tih vrsta.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:667753
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-02-27
Terms of useLicence In copyright icon
Created on2020-06-09 10:14:39