Macrozoobenthos community represents an important component within biocenotic structures and nutrient cycles, and it is an important part of food webs in freshwaters. Any kind of pollution of freshwater affects the composition and structure of macrozoobenthos, so they are good indicators of water quality. The aim of this research is to determine the water quality status of the Lištica and Radobolja Rivers, based on the analysis of the composition and structure of the macrozoobenthos community and to determine the spatial differences between the sites in the rivers and among the rivers. Samples were collected through two seasons, spring / summer and summer / fall, at three sites in the Lištica River along the longitudinal profile, and at four sites in the Radobolja River from the source to the confluence with the Neretva River. In the laboratory isolation and identification of collected macrozoobenthos samples was conducted and the analysis of the macrozoobenthos community. The results obtained show the high to moderate ecological status of the Lištica and Radobolja rivers. The obtained results have shown the high ecological status of the rivers Lištica and Radobolja. The Radobolja River has shown the greater diversity and abundance of macrozoobenthos. The composition and structure of macrozoobenthos in Lištica River is similar at all sites. In the Radobolja River site R1, which is the spring is different from the sites R4 since this sites is under great anthropogenic influence. Biomonitoring of these rivers is important and it needs to be regularly conducted because the Lištica and Radobolja Rivers are used for water supply of the cities Mostar and Široki Brijeg.