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master's thesis
Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali'
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:370503
Norac - Kljajo, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Norac Kljajo, J. (2014). Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali' (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:370503

Norac Kljajo, Jelena. "Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali'." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:370503

Norac Kljajo, Jelena. "Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali'." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:370503

Norac Kljajo, J. (2014). 'Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali'', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:370503

Norac Kljajo J. Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali' [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:370503

J. Norac Kljajo, "Multigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali'", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:370503

Metadata
TitleMultigenska tipizacija izolata bakterije 'Candidatus Phytoplasma mali'
Title (english)Multigene sequence analysis of 'Candidatus Phytoplasma mali' isolates
AuthorJelena Norac - Kljajo
MentorMartina Šeruga Musić (mentor)
Committee memberMartina Šeruga Musić (predsjednik povjerenstva)
Committee memberVesna Petrović Peroković (član povjerenstva)
Committee memberInes Radanović (član povjerenstva)
Committee memberZora Popović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitoplazme (rod 'Candidatus Phytoplasma') su bakterijski patogeni koji uzrokuju brojne ekonomski značajne bolesti biljaka u čitavom svijetu. Zbog svoje male veličine, izostanka stanične stijenke i nemogućnosti uzgajanja u čistoj kulturi in vitro svrstane su u razred Mollicutes. Fitoplazme imaju iznimno reducirane genome (680 - 1600 kb) i nedostaju im mnogi esencijalni geni za različite metaboličke putove. U svom životnom ciklusu obavezno izmjenjuju dva domaćina – biljku i kukca koji je ujedno i vektor. Dok kod kukaca najčešće ne izazivaju nikakve smetnje, njihov učinak na biljke je često vrlo štetan. U biljkama se zadržavaju unutar floema što im osigurava unos potrebnih metabolita za preživljavanje i umnažanje. Proliferacija jabuke (apple proliferation; AP) jedna od ekonomski značajnih bolesti jabuke u Europi, a glavni uzročnik je fitoplazma vrste 'Ca. Phytoplasma mali'. Najčešći karakteristični simptomi su proliferacija aksilarnih pupova, rast u obliku vještičje metle, crvenjenje i žućenje listova s nekrozom floema te maleni plodovi loše boje i kvalitete. U Hrvatskoj prisutnost ovog uzročnika tek je nedavno po prvi puta dokazana molekularnim metodama. U ovom istraživanju umnažanjem i restrikcijskom analizom gena za 16S rRNA dokazana je prisutnost ove vrste u uzorcima prikupljenim na simptomatičnim stablima jabuka s više lokacija u Hrvatskoj te jednog uzorka kukca-vektora iz roda Cacopsylla. Multigenska tipizacija izolata obuhvatila je analizu gena aceF i pnp umnažanjem metodom PCR korištenjem specifičnih početnica te sekvenciranjem dobivenih produkata. Filogenetskom analizom nukleotidnih sljedova gena aceF i pnp dobiveni su korisni podaci o raznolikosti izolata bakterije 'Ca. Phytoplasma mali u Hrvatskoj koja je po prvi puta tipizirana.
Abstract (english)
Phytoplasma (genus 'Candidatus Phytoplasma') are bacterial pathogens that cause numerous diseases in plants. Because of their small size, lack of cell wall and the inability of cultured in vitro they are affiliated to the class Mollicutes. Phytoplasmas have extremely reduced genome (680 - 1600 kb) and lack many essential genes for different metabolic pathways. Life cycle of phytoplasma involves two hosts – plant and insects. Although in insects usually there usually do not cause negative effects, their effect on plants is often fatal. Attacking the plant cells phytoplasmas retain within phloem from which metabolites are taken directly for growth and multiplication. Species ' Ca. Phytoplasma mali' causes apple proliferation (AP) and it is one of the most economically important diseases of fruit trees in Croatia. The most common characteristic symptoms of AP are proliferation of axillar buds, growth of branches known as ''witches' broom'', yellowing or reddening of leaves with phloem necrosis together with small, broadly shaped and poorly flavored fruit. Amplification and RFLP analysis of 16S rRNA gene confirmed the presence of 'Ca. Phytoplasma mali' in samples collected from symptomatic apple trees and samples from insect-vector. From positive samples aceF and pnp genes were amplified by PCR using specific primers and sent for sequencing. Phylogenetic analyses of obtained nucleotide sequences revealed useful information about variability of ‘Ca. Phytoplasma mali’ isolates in Croatia and represent the first genotyping study of this pathogen in the country.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:370503
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 10:50:57