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master's thesis
Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:831200
Manenica, Martina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

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Manenica, M. (2020). Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:831200

Manenica, Martina. "Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:831200

Manenica, Martina. "Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:831200

Manenica, M. (2020). 'Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:831200

Manenica M. Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:217:831200

M. Manenica, "Biofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:831200

Metadata
TitleBiofizička svojstva proteina regulatora citokineze 1, PRC1, u fazama mitoze ljudskih stanica
Title (english)Biophysical properties of the protein regulator of cytokinesis 1, PRC1, in the phases of the human cell mitosis
AuthorMartina Manenica
MentorIva Tolić (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberBranimir Bertoša (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Stanična dioba je efikasan je i pouzdan proces koji omogućuje raspodjelu genetičkog i staničnog materijala. Brojni proteini koji omogućuju i reguliraju staničnu diobu vezani su na diobeno vreteno. Jedan od njih je i protein regulator citokineze 1, PRC1. U ovom radu proučavana su biofizička svojstva PRC1 fuzioniranog na zeleni fluorescentni protein, GFP, metodom oporavka fluorescencije nakon fotoizblijeđenja (FRAP) u ljudskim stanicama. Od početka do kraja diobe afinitet vezanja PRC1-GFP na vreteno se izrazito mijenja. Prilagođavanjem kinetičkog modela na eksperimentalne podatke, pokazano je da je PRC1- GFP na početku diobe mobilan protein koji se prema kraju veže na vreteno sve jačim afinitetom. Na ovaj način dobivene su pouzdane informacije o kinetičkim svojstvima PRC1- GFP u nativnim uvjetima žive stanice.
Abstract (english)
Cell division is efficient and precise process which enables the distribution of genetic and cellular material. Numerous proteins that enable and regulate cell division are associated with the microtubule structure called mitotic spindle. One of them is the protein regulator of cytokinesis 1, PRC1. Biophysical properties of PRC1 fused to green fluorescent protein, GFP, were investigated by recovery of fluorescence after photobleaching (FRAP) in human cells. From the beginning to the end of the division, the binding affinity of PRC1-GFP to the spindle changes markedly. By adjusting the kinetical model on the experimental data, it was shown that PRC1-GFP at the beginning of division is a mobile protein loosely bound to the spindle. As the division progresses, binding affinity increases. In this way, reliable information on the kinetic properties of PRC1-GFP was gained in real time in live cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:831200
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-07-09
Terms of useLicence In copyright icon
Created on2020-07-20 09:55:48