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master's thesis
Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:045746
Hubak, Ana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Hubak, A. (2020). Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:045746

Hubak, Ana. "Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:045746

Hubak, Ana. "Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:045746

Hubak, A. (2020). 'Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:045746

Hubak A. Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:045746

A. Hubak, "Utjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:045746

Metadata
TitleUtjecaj polistirenskih i srebrnih nanočestica na vijabilnost i oksidativni stres modelnih staničnih linija Jurkat, HepG2
Title (english)The impact of polystyrene and silver nanoparticles on the viability and oxidative stress of Jurkat AND HepG2 cell lines
AuthorAna Hubak
MentorIvan Pavičić (mentor)
MentorSandra Radić Brkanac (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Jurkat stanična linija je besmrtna stanična linija T-limfocita koja je izvorno dobivena iz periferne krvi četrnaestogodišnjaka oboljelog od akutne limfoblastične leukemije, a HepG2 stanična linija je epitelna stanična linija koja je dobivena iz jetre hepatocelularnog karcinoma petnaestogodišnjeg dječaka. Obje stanične linije korištene su kao modelne stanične linije za ispitivanje toksičnosti nanočestica polistirena i srebra koje su bile stabilizirane različitim omotačima. Osim određivanja preživljavanja staničnih linija nakon tretmana s nanočesticama srebra i polistirena, mjerili smo i razine biomarkera oksidativnog stresa primjenom fluorescentnih proba diklorodihidroflourescein diacetata i monoklorobimana. Rezultati dobiveni metodom Live/Dead na protočnom citometru pokazuju da se kombinirani tretman nanočestica na Jurkat staničnoj liniji pokazao citotoksičnijim od pojedinačnih tretmana s istim koncentracijama nanočestica. Dobiveni rezultati također pokazuju da je Jurkat stanična linija osjetljivija od HepG2 stanične linije. Nakon tretmana nanočesticama srebra i polistirena mjeren je ukupan sadržaja glutationa (GSH) i reaktivnih kisikovih vrsta u staničnim linijama Jurkat i HepG2 te nije dokazana pojava oksidativnog stresa.
Abstract (english)
Jurkat cell lines are immortalized T-lymphocytes first derived from the peripheral blood of a child suffering from leukemia. HepG2 cell line is an immortalized cell line of human liver carcinoma cells, derived from the liver tissue of child suffering from hepatocellular carcinoma. In this research, we used cell lines as a test organism to assess toxicity of polystyrene and silver nanoparticles stabilized with different biomolecules. Besides determining the survival of cell lines after the treatment with polystyrene and silver nanoparticles we, also measured levels of oxidative stress biomarkers by using fluorescent probes dichloro-dihydro-fluorescein diacetate and monochlorobimane. Results with Live/Dead flow cytometry tests demonstrated that combined treatment of Jurkat cells with both PVPAgNP and PSNPs has proven to be more cytotoxic than individual treatments with the same nanoparticle concentrations. Obtained results also show that Jurkat cell line proved to be more sensitive than the HepG2 cell line. Oxidative stress response in cells was detected by measuring total amount of reactive oxygen species and glutathione. PVPAgNP and PSNP did not trigger oxidative in Jurkat and HepG2 cell lines.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:045746
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-07-20 11:24:41