prikaz prve stranice dokumenta Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly
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Scientific paper - Original scientific paper
Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly
BMC Biology, 17 (2019), 1; 42. https://doi.org/10.1186/s12915-019-0656-2
Winters, Lora; Ban, Ivana; Prelogović, Marcel; Kalinina, Iana; Pavin, Nenad; Tolić, Iva M.

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Winters, L., Ban, I., Prelogović, M., Kalinina, I., Pavin, N. & Tolić, I. M. (2019). Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly. BMC Biology, 17. (1). doi: 10.1186/s12915-019-0656-2

Winters, Lora, et al. "Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly." BMC Biology, vol. 17, no. 1, 2019. https://doi.org/10.1186/s12915-019-0656-2

Winters, Lora, Ivana Ban, Marcel Prelogović, Iana Kalinina, Nenad Pavin and Iva M. Tolić. "Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly." BMC Biology 17, no. 1 (2019). https://doi.org/10.1186/s12915-019-0656-2

Winters, L., et al. (2019) 'Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly', BMC Biology, 17(1). doi: 10.1186/s12915-019-0656-2

Winters L, Ban I, Prelogović M, Kalinina I, Pavin N, Tolić IM. Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly. BMC Biology [Internet]. 2019 [cited 2024 November 04];17(1). doi: 10.1186/s12915-019-0656-2

L. Winters, I. Ban, M. Prelogović, I. Kalinina, N. Pavin and I. M. Tolić, "Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly", BMC Biology, vol. 17, no. 1, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:916658. [Accessed: 04 November 2024]

Metadata
Title (english)Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly
AuthorLora Winters
AuthorIvana Ban
AuthorMarcel Prelogović
AuthorIana Kalinina
AuthorNenad Pavin
AuthorIva M. Tolić
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
Background. At the beginning of mitosis, the cell forms a spindle made of microtubules and associated proteins to segregate chromosomes. An important part of spindle architecture is a set of antiparallel microtubule bundles connecting the spindle poles. A key question is how microtubules extending at arbitrary angles form an antiparallel interpolar bundle. Results. Here, we show in fission yeast that microtubules meet at an oblique angle and subsequently rotate into antiparallel alignment. Our live-cell imaging approach provides a direct observation of interpolar bundle formation. By combining experiments with theory, we show that microtubules from each pole search for those from the opposite pole by performing random angular movement. Upon contact, two microtubules slide sideways along each other in a directed manner towards the antiparallel configuration. We introduce the contour length of microtubules as a measure of activity of motors that drive microtubule sliding, which we used together with observation of Cut7/kinesin-5 motors and our theory to reveal the minus-end-directed motility of this motor in vivo. Conclusion. Random rotational motion helps microtubules from the opposite poles to find each other and subsequent accumulation of motors allows them to generate forces that drive interpolar bundle formation.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBMC Biology
Numberingvol. 17, no. 1, 42
e-ISSN1741-7007
DOIhttps://doi.org/10.1186/s12915-019-0656-2
URN:NBNurn:nbn:hr:217:916658
Publication2019
Document URLhttps://bmcbiol.biomedcentral.com/articles/10.1186/s12915-019-0656-2
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© The Author(s). 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Published: 23 May 2019.
Created on2020-07-24 13:45:33