prikaz prve stranice dokumenta Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma
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master's thesis
Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma
Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:781702
Slović, Anamarija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Slović, A. (2014). Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:781702

Slović, Anamarija. "Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:781702

Slović, Anamarija. "Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:781702

Slović, A. (2014). 'Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:781702

Slović A. Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:781702

A. Slović, "Raznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:781702

Metadata
TitleRaznolikost gena za molekularni šaperonin GroEL u genomima bakterija roda Candidatus Phytoplasma
Title (english)Diversity of genes encoding molecular chaperonin GroEL in the genomes of bacteria from the genus Candidatus Phytoplasma
AuthorAnamarija Slović
MentorMartina Šeruga Musić (mentor)
Committee memberMartina Šeruga Musić (predsjednik povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2014-01-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitoplazme ('Candidatus Phytoplasma') su bakterije bez stanične stijenke iz razreda Mollicutes. Obligatni su simbionti biljaka i kukaca te uzrokuju brojne ekonomski značajne bolesti mnogih poljoprivrednih kultura u cijelome svijetu. Nemoguće ih je uzgajati u mediju bez stanica, čime je njihova detekcija i klasifikacija otežana. Molekularni šaperonin GroEL pomaže u smatanju drugih proteina, a prisustvo ovog gena detektirano je u svim potpuno sekvenciranim genomima fitoplazmi. Zbog mnogobrojnih uloga proteina GroEL, ovaj je gen istražen u novim izolatima fitoplazmi ribosomske podskupine 16SrXII-A, koje pripadaju vrsti 'Ca. P. solani' i uzrokuju ekonomski značajne štete vinove loze (Vitis vinifera L.). Gen za molekularni šaperonin GroEL iz nekoliko izolata uspješno je umnožen PCR-om, nakon čega je određen slijed nukleotida ovog gena, a metodom Southern blot detektirana je jedna kopija gena u genomu. Raznolikost gena novih izolata i gena iz poznatih genoma ispitana je filogenetskim analizama, koje su pokazale očekivano grupiranje fitoplazmi s obzirom na 16Sr skupine, što ovaj gen čini pogodnim markerom za analizu evolucijskih odnosa među fitoplazmatskim vrstama kao i varijabilnost izolata unutar pojedine vrste.
Abstract (english)
Phytoplasmas ('Candidatus Phytoplasma') are wall-less bacteria from the class Mollicutes. They are obligate symbionts of plants and insects and cause diseases in numerous agriculturally significant plant species. It is impossible to cultivate them in cell-free media, making their detection and classification difficult. Molecular chaperonin GroEL assists in the folding of other proteins, and its presence was detected in all phytoplasma genomes that have been sequenced to completion. Because of many various functions of GroEL protein, this gene was studied in new phytoplasma isolates from the 16SrXII-A ribosomal subgroup, belonging to the 'Ca. P. solani' species that cause significant economic damage of the grapevine (Vitis vinifera L.). Gene encoding molecular chaperonin GroEL was successfully amplified by PCR from several isolates, and subsequently nucleotide sequence of the gene was determined. The presence of a single copy of the gene in the genome was confirmed by Southern blot method. Phylogenetic analysis of groEL gene sequences belonging to new phytoplasma isolates and those available in GenBank was also performed. Considering the 16S rDNA gene based groups, the expected phytoplasma grouping was observed, making this gene a suitable marker for analysis of evolutionary relationships among different phytoplasma species as well as the studies of intraspecies variability.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:781702
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 12:24:23