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undergraduate thesis
Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:632140
Lalić, Dora
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Lalić, D. (2020). Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:632140

Lalić, Dora. "Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu." Undergraduate thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:632140

Lalić, Dora. "Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu." Undergraduate thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:632140

Lalić, D. (2020). 'Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:632140

Lalić D. Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:632140

D. Lalić, "Uloga sustava CRISPR/Cas9 u genetičkom injženjerstvu", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:632140

Metadata
TitleUloga sustava CRISPR/Cas9 u genetičkom injženjerstvu
Title (english)CRISPR/Cas9 system in genetic engineering
AuthorDora Lalić
MentorVlatka Zoldoš (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-08-31, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Promjene u nukleotidnoj sekvenci DNA, kao i epigenetičke promjene koje rezultiraju perturbacijom kromatinske strukture, mogu dovesti do razvitka mnogih bolesti u ljudi i životinja. Otkriće sustava CRISPR/Cas9 koji potječe od prokariotskih organizama u kojima ima ulogu imunosnog sustava, omogućilo je determinaciju i istraživanje kodirajuće i nekodirajuće DNA, aktivaciju ili represiju genske aktivnosti te uvođenje mjesno-specifičnih mutacija u sve tri domene života. Sustav CRISPR/Cas9 predstavlja precizan i efikasan alat za ciljano uređivanje genoma i manipulaciju genske ekspresije. Lakoća programibilnosti ovog dvokomponentnog sustava promjenom kratke nukleotidne sekvence u molekuli RNA da prepoznaje virtualno bilo koje mjesto unutar genoma, daje ovoj tehnologiji veliki potencijal za primjenu u genetičkom injženjerstvu, biotehnologiji te istraživanju, liječenju i prevenciji bolesti uzrokovanih genetičkim ili epigenetičkim promjenama.
Abstract (english)
Changes in the nucleotide sequence of DNA, as well as epigenetic changes which result in chromatin perturbation, can lead to development od many diseases in both animals and humans. Discovery of CRISPR/Cas9 system derived from prokaryotes, in which it serves as an adaptive immune system, enables determination and investigation od both coding and noncoding DNA, activation or repression of certain genes and introduction of site-specific mutations in all three domains of life. CRISPR/Cas9 system is a precise and efficient tool for targeted genome editing and manipulation of gene expression. Because this two-component system is so easily programmable by altering short nucleotide sequence of an RNA molecule to target virtually any locus within the genome, it holds great potential for application in genetic engineering, biotechnology and investigation, treatment and prevention of diseases caused by genetic or epigenetic changes.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:217:632140
Study programmeTitle: Ecology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-09-09 12:29:44