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master's thesis
Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:521309
Domitrović, Lidija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Domitrović, L. (2013). Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:521309

Domitrović, Lidija. "Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:521309

Domitrović, Lidija. "Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:521309

Domitrović, L. (2013). 'Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:521309

Domitrović L. Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:521309

L. Domitrović, "Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:521309

Metadata
TitleZajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora
Title (english)The effect of simultaneous treatment with ochratoxin A and citrinin on oxidative stress in rat
AuthorLidija Domitrović
MentorMaja Peraica (mentor)
MentorNada Oršolić (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberMaja Peraica (član povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberVesna Benković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-02-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Mikotoksini okratoksin A (OTA) i citrinin (CIT) često su istovremeno prisutni u uzorcima hrane. Uz dokazano nefrotoksično, hepatotoksično i karcinogeno djelovanje, povezuju se s bolešću balkanska endemska nefropatija (BEN). Mehanizam njihovog staničnog djelovanja neki autori povezuju sa stvaranjem oksidacijskog stresa. Cilj ovog istraživanja bio je utvrditi je li oksidacijski stres mehanizam toksičnog učinka OTA i CIT na pokusnim životinjama, te mijenja li se taj učinak ako su primijenjeni istovremeno. Istraživanje je provedeno na odraslim mužjacima štakora soja Wistar. U skupini izlaganoj OTA (250 μg/kg t.m. dnevno, kroz tri tjedna) izmjereno je značajno smanjenje koncentracije reduciranog glutationa (GSH) u bubrezima te povećanje koncentracije malondialdehida (MDA) u jetri. U skupini izlaganoj CIT (20 mg/kg t.m., dva dana) nije bilo značajnih promjena. U skupini izlaganoj OTA i CIT (250 μg/kg t.m. OTA dnevno, kroz tri tjedna + 20 mg/kg t.m. CIT zadnja dva dana) izmjereno je značajno smanjenje koncentracije GSH u mozgu te povećanje koncentracije MDA u jetri i bubrezima. Primijećen je aditivan učinak OTA i CIT na povećanje koncentracije MDA u jetri. Takvi rezultati ukazuju na moguću zajedničku ulogu OTA i CIT prilikom izazivanja BEN.
Abstract (english)
Mycotoxins ochratoxin A (OTA) and citrinin (CIT) are often simultaneously present in food samples. Due to their nephrotoxic, hepatotoxic and carcinogenic effects, they are brought in connection with a disease called Balkan endemic nephropathy (BEN). Mechanism of their cellular activity is related with formation of oxidative stress by some authors. The objective of this research was to determine if oxidative stress is a mechanism of toxic effect of OTA and CIT on experimental animals, and whether that effect changes if they are applied simultaneously. The research was conducted on male Wistar rats. In the group treated with OTA (250 µg/kg b.m. per day, for three weeks), a significant decrease in glutathione (GSH) concentration in kidneys and increase of malondialdehyde (MDA) concentration in liver were measured. In the group treated with CIT (20 mg/kg b.m., two days) there were no significant changes. In the group treated with OTA and CIT (250 µg/kg b.m. OTA per day, for three weeks + 20 mg/kg b.m. CIT last two days) a significant decrease in GSH concentration in brain and increase of MDA concentration in liver and kidneys were found. Additive effect of OTA and CIT on increase of MDA concentration in liver was noticed. Such results indicate to the possible mutual role of OTA and CIT in causing BEN.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:521309
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 07:38:06