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master's thesis
Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel
Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:048074
Ferenčak, Krešimir
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Ferenčak, K. (2013). Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:048074

Ferenčak, Krešimir. "Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:048074

Ferenčak, Krešimir. "Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:048074

Ferenčak, K. (2013). 'Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:048074

Ferenčak K. Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:048074

K. Ferenčak, "Utjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:048074

Metadata
TitleUtjecaj utišavanja integrina alfa-v ili alfa-4 na osjetljivost stanica tumora dojke na vinkristin i paklitaksel
Title (english)Integrin alfa-v or alfa-4 silencing sensitizes human breast carcinoma cell line MDA-MB-435S to vincristine and paclitaxel but has differential effect on cell migration
AuthorKrešimir Ferenčak
MentorAndreja Ambriović Ristov (mentor)
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberAndreja Ambriović Ristov (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2013-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Integrini su transmembranski heterodimerni proteini koji posreduju u prihvaćanju stanica za druge stanice i izvanstanični matriks. Osim što sudjeluju u regulaciji proliferacije, migracije, metastaziranja i angiogeneze, reguliraju i osjetljivost stanica na protutumorske lijekove. U ovom radu smo odredili učinak utišavanja podjedinica integrina αv, α4, α3, ß3 i ß5 pomoću integrin specifičnih siRNA u stanicama tumora dojke MDA-MB-435S na osjetljivost na protutumorske lijekove koji se koriste u liječenju tumora dojke. Utišavanjem podjedinica αv, α3, ß3 i ß5 smanjuje se osjetljivost stanica MDA-MB-435S na cisplatinu, dok se ne mijenja osjetljivost na doksorubicin i kamptotecin. Međutim, utišavanje podjedinica αv, α4 i ß5 povećava osjetljivost stanica na vinkristin i paklitaksel. Također je pokazano da se sposobnost migracije stanica smanjuje nakon utišavanja podjedinice integrina αv, a povećava nakon utišavanja α4. Ovi rezultati upućuju da bi transfekcija stanica tumora dojke koje eksprimiraju integrine αv, αv-specifičnom siRNA mogla povećati djelotvornost vinkristina i paklitaksela i smanjiti metastatski potencijal.
Abstract (english)
Integrins are heterodimeric transmembrane proteins that mediate the adhesion of cells to other cells and extracellular matrix. Besides regulation of proliferation, migration, metastasis and angiogenesis, integrins regulate cell sensitivity to antitumor drugs. In this study we determine the effect of integrin subunits αv, α4, α3, ß3 and ß5 silencing using integrin-specific siRNA in MDA-MB-435S breast cancer cells in sensitivity to antitumor drugs used in the treatment of breast cancer. αv, α3, ß3 and ß5 subunit silencing reduces sensitivity of cells to cisplatin, while not changing the sensitivity to doxorubicin and kamptotecin. However, αv, α4 and ß5 silencing increases cell sensitivity to vincristine and paclitaxel. It was also shown that the ability of cell migration reduces after αv silencing, and increases after α4 silencing. These results indicate that transfection of breast cancer cells expressing αv integrins with αv-specific siRNA could increase the effectiveness of vincristine and paclitaxel, and at the same time reduce the metastatic potential.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:048074
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 07:48:15