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master's thesis
Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:490054
Kodba, Snježana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kodba, S. (2020). Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:490054

Kodba, Snježana. "Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:490054

Kodba, Snježana. "Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:490054

Kodba, S. (2020). 'Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva', Master's thesis, University of Zagreb, Faculty of Science, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:490054

Kodba S. Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:490054

S. Kodba, "Uspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:490054

Metadata
TitleUspostava kulture i biološko oslikavanje organoida mišjeg tankog crijeva
Title (english)Establishment and bioimaging of mouse small intestine organoids culture
AuthorSnježana Kodba
MentorIva Marija Tolić (mentor)
Committee memberIva Marija Tolić (predsjednik povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberAna Previšić (član povjerenstva)
Committee memberInga Urlić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2020-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Organoidi su novo područje u molekularnoj biologiji koje ima veliki potencijal u temeljnim i primijenjenim istraživanjima. Minijaturni organi uzgajani in vitro značajno nalikuju svojim nativnim dvojnicima u svim fiziološkim, 3D strukturalnim i anatomskim karakteristikama. Već je razvijeno više tipova organoida, uključujući ljudske i mišje organoide tankog crijeva. U ovom radu, ja sam uspješno uzgojila i održala u kulturi organoide mišjeg tankog crijeva. Nakon optimizacije, uzgojeni su organoidi mogli neodređeno dugo rasti i propagirati se u kulturi. Preciznija karakterizacija anatomije i morfologije organoida napravljena je nakon imunofluorescencijskog bojanja i biološkog oslikavanja. Nadalje, razvila sam novi protokol za imunofluorescencijsko bojanje cističnih, šupljih tipova organoida. U odnosu na literaturno dostupne protokole, novi protokol predstavlja poboljšanja u brzini izvedbe i broju koraka koji štede vrijeme i novac. Također, rezultati su pokazali da organoidi mišjeg tankog crijeva mogu dolaziti u različitim oblicima pri čemu veličina stanica unutar organoida ovisi o točnoj poziciji stanice u organoidu te o njihovom proliferacijskom statusu. Na kraju, stopa proliferacije organoida mišjeg tankog crijeva pada s povećanjem broja dana koje su organoidi proveli u kulturi. Zaključno, uspješno je razvijen protokol za izolaciju i uzgoj složenog sustava organoida iz mišjeg tankog crijeva koji ima potencijal brze optimizacije za korištenje na organoidima drugih organskih sustava.
Abstract (english)
A novel field arising in molecular biology, organoids, have a great potential in basic and applied research. Mini organs grown in a dish remarkably resemble their native counterparts in almost every aspect of their physiological, 3D structural and anatomical properties. Many different types of organoids have yet been developed, including mouse and human small intestine organoids. In this thesis, I have successfully established and maintained a culture of mouse small intestine organoids. After some optimization was done, the organoids were able to keep growing and differentiating indefinitely. More precise characterization of their anatomy and morphology was made after immunofluorescent staining and bio-imaging. I have created a novel protocol for immunostaining of cystic, hollow types of the organoids which reduces the time and money consumed. The results have revealed different shapes of small intestine organoids and variation in cell size depending on the position and proliferation status. The proliferation rate, however, decreases as the days in culture increase. To conclude, a novel protocol for isolation and propagation of complex organoid system was successfully developed from mouse small intestine. However, it has potential for fast optimization and adjustment for other types of mouse and human organoids.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:490054
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-24
Terms of useLicence In copyright icon
Created on2020-10-02 09:04:13