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master's thesis
Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka
Zagreb: University of Zagreb, Faculty of Science, 2020. urn:nbn:hr:217:789551
Besednik, Lucija
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Besednik, L. (2020). Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:789551

Besednik, Lucija. "Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:789551

Besednik, Lucija. "Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka." Master's thesis, University of Zagreb, Faculty of Science, 2020. https://urn.nsk.hr/urn:nbn:hr:217:789551

Besednik, L. (2020). 'Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka', Master's thesis, University of Zagreb, Faculty of Science, accessed 13 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:789551

Besednik L. Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2020 [cited 2024 July 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:789551

L. Besednik, "Mikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:217:789551

Metadata
TitleMikrovalno potpomognuta ekstrakcija polibromiranih difenil etera iz ljudskog mlijeka
Title (english)Microvawe-assisted extraction of polybrominated diphenyl ethers from human milk
AuthorLucija Besednik
MentorDarija Klinčić (mentor)
Committee memberNives Galić (predsjednik povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2020-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Razrađeni su uvjeti ekstrakcije potpomognute mikrovalovima (MAE) za polibromirane difenil etere (PBDE) iz uzoraka ljudskog mlijeka. Istražen je utjecaj načina pripreme uzorka te su optimirani uvjeti tehnike MAE (vrsta i volumen otapala, temperatura ekstrakcije). Pročišćeni ekstrakti analizirani su plinskom kromatografijom uz detektor zahvata elektrona. Tehnika MAE pokazala je zadovoljavajuću djelotvornost za ekstrakciju spojeva PBDE iz uzoraka ljudskog mlijeka u tekućem stanju, dok u slučaju liofiliziranog uzorka mlijeka nije bila djelotvorna. Postupak ekstrakcije spojeva PBDE tehnikom MAE iz liofiliziranog ljudskog mlijeka otopljenog u ultračistoj vodi smjesom otapala φ (n-heksan, aceton) = 1:1 uz dodatak mravlje kiseline i propan-2-ola pri temperaturi od 80 °C tijekom 20 minuta izdvojen je kao najprikladniji. Analitički povrat spojeva bio je u rasponu od 61 % za BDE-47 do 83 % za BDE-183, uz relativnu standardnu devijaciju 7 %. U uzorku korištenom za razradu metode analiziranom bez dodatka analita pri odabranim uvjetima detektiran je samo BDE-153 (0,19 ng g–1 masti).
Abstract (english)
Microwave-assisted extraction (MAE) procedures for polybrominated diphenyl ethers (PBDEs) from human milk samples have been studied. The influence of the sample preparation method was investigated and the conditions of the MAE technique (type and volume of solvent, extraction temperature) were optimized. The purified extracts were analyzed by gas chromatography with an electron capture detector. The MAE technique has shown satisfactory efficiency for the extraction of PBDEs from liquid milk samples, while in the case of a lyophilized milk sample it was not effective. The process of extraction of PBDEs by MAE technique from lyophilized human milk dissolved in ultrapure water with a mixture of solvent φ (n-hexane, acetone) = 1: 1 with the addition of formic acid and propan-2-ol at a temperature of 80 ° C for 20 minutes was selected as the most suitable. Analytical recoveries of selected analytes ranged from 61 % for BDE-47 to 83 % for BDE-183, with a relative standard deviation of 7 %. In the sample used for method development, analyzed without the addition of analytes under selected conditions, only BDE-153 was detected (0,19 ng g–1 lipid).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:789551
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-18
Terms of useLicence In copyright icon
Created on2020-10-07 08:57:08